Comparison of ionised calcium measured using a portable analyser to a reference method in healthy dogs

Harper, J.A., Gal, A., Burchell, R.K., Summers, J.D., Starling, J., Gerber, K., and Gummow, B. (2023) Comparison of ionised calcium measured using a portable analyser to a reference method in healthy dogs. Journal of Small Animal Practice, 64 (5). pp. 337-342.

[img] PDF (Accepted Publisher Version) - Accepted Version
Restricted to Repository staff only

View at Publisher Website:


Objectives: To compare the ionised calcium measured on a portable analyser (iSTAT, Abbott) to a reference method.

Materials and Methods: Blood samples from 39 apparently healthy dogs were analysed in duplicate using a portable analyser and a reference method (Radiometer ABL800 FLEX). Bland–Altman plots and Passing–Bablok regression were used to assess constant and proportional bias between the two instruments. A within-assay percentage coefficient of variation and total error (TE) was calculated for both analysers. The reference interval was calculated for the portable analyser using the robust method with confidence interval bootstrapping.

Results: The Bland–Altman plot showed a −0.036 mmol/L difference between the two instruments (95% confidence limit −0.08 to 0.01 mmol/L; limits of agreement −0.07 to 0.006 mmol/L). Neither the Bland–Altman plot nor the Passing–Bablock regression (slope −0.03; 95% confidence interval −0.08 to 0.19 and intercept 1; 95% confidence interval 0.83 to 1.2) showed significant proportional bias. The coefficient of variation for the portable analyser was 1.08%, compared to 0.78% for the reference method with a total error of 3.5% for the portable analyser. The estimated population-based reference interval for ionised calcium using the portable analyser is 1.23 to 1.42 mmol/L.

Clinical Significance: For the healthy dogs in this study, compared to the reference method, the portable analyser showed no significant bias for measurement of ionised calcium. Further studies including hyper and hypocalcaemic dogs are required to determine clinical impact of the use of this analyser.

Item ID: 77931
Item Type: Article (Research - C1)
ISSN: 1748-5827
Copyright Information: © 2023 British Small Animal Veterinary Association.
Date Deposited: 22 Mar 2023 02:02
FoR Codes: 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3009 Veterinary sciences > 300910 Veterinary pathology @ 100%
SEO Codes: 28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280101 Expanding knowledge in the agricultural, food and veterinary sciences @ 100%
Downloads: Total: 1
Last 12 Months: 1
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page