Application of Autologous Platelet-Rich Plasma on Tooth Extraction Site Prevents Occurence of Medication-Related Osteonecrosis of the Jaws in Rats
Toro, Luan Felipe, de Mello-Neto, João Martins, Santos, Fernanda Furuse Ventura dos, Ferreira, Letícia Chaves, Statkievicz, Cristian, Cintra, Luciano Tavares Ângelo, Issa, João Paulo Mardegan, Dornelles, Rita Cássia Menegati, de Almeida, Juliano Milanezi, Nagata, Maria José Hitomi, Garcia, Valdir Gouveia, Theodoro, Leticia Helena, Casatti, Cláudio Aparecido, and Ervolino, Edilson (2019) Application of Autologous Platelet-Rich Plasma on Tooth Extraction Site Prevents Occurence of Medication-Related Osteonecrosis of the Jaws in Rats. Scientific Reports, 9. 22.
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Abstract
This study evaluated the effects of local application of autologous platelet-rich plasma (PRP) on the tooth extraction site of rats presenting the main risk factors for medication-related osteonecrosis of the jaw (MRONJ). For seven weeks, senile rats were submitted to systemic treatment with vehicle (VEH and VEH-PRP) or 100 μg/Kg of zoledronate (ZOL and ZOL-PRP) every three days. After three weeks, the first lower molar was extracted. VEH-PRP and ZOL-PRP received PRP at the tooth extraction site. Euthanasia was performed at 28 days postoperatively. Clinical, histopathological, histometric and immunohistochemical analyses were carried out in histological sections from the tooth extraction site. ZOL showed lower percentage of newly formed bone tissue (NFBT), higher percentage of non-vital bone tissue (NVBT), as well as higher immunolabeling for TNFα and IL-1β. In addition, ZOL presented lower immunolabeling for PCNA, VEGF, BMP2/4, OCN and TRAP. VEH and ZOL-PRP showed improvement in the tooth extraction site wound healing and comparable percentage of NFBT, VEGF, BMP2/4 and OCN. Local application of autologous PRP proved a viable preventive therapy, which is safe and effective to restore tissue repair capacity of the tooth extraction site and prevent the occurrence of MRONJ following tooth extraction.
| Item ID: | 77861 |
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| Item Type: | Article (Research - C1) |
| ISSN: | 2045-2322 |
| Copyright Information: | © The Author(s) 2019. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| Date Deposited: | 14 Mar 2023 23:47 |
| FoR Codes: | 32 BIOMEDICAL AND CLINICAL SCIENCES > 3203 Dentistry > 320301 Craniofacial biology @ 33% 32 BIOMEDICAL AND CLINICAL SCIENCES > 3203 Dentistry > 320307 Oral medicine and pathology @ 34% 32 BIOMEDICAL AND CLINICAL SCIENCES > 3203 Dentistry > 320303 Dental therapeutics, pharmacology and toxicology @ 33% |
| SEO Codes: | 20 HEALTH > 2001 Clinical health > 200104 Prevention of human diseases and conditions @ 50% 20 HEALTH > 2001 Clinical health > 200105 Treatment of human diseases and conditions @ 50% |
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