De Brauwer, Maarten, Clarke, Laurence J., Chariton, Anthony, Cooper, Madalyn K., De Bruyn, Mark, Furlan, Elise, Macdonald, Anna J., Rourke, Meaghan L., Sherman, Craig D.H., Suter, Leonie, Villacorta Rath, Cecilia, Zaiko, Anastasija, and Trujillo-González, Alejandro (2023) Best practice guidelines for environmental DNA biomonitoring in Australia and New Zealand. Environmental DNA, 5 (3). pp. 417-423.

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Abstract

Environmental DNA (eDNA)- based methods are increasingly used by government agencies to detect pests and threatened species, and for broader biodiversity monitor-ing. Given rapid technological advances and a growing number of commercial service providers, there is a need to standardize methods for quality assurance and to main-tain confidence in eDNA- based results. Here, we introduce two documents to pro-vide best- practice guidelines for Australian and New Zealand eDNA researchers and end- users (available from https://sedna socie ty.com/publications ): the Environmental DNA protocol development guide for biomonitoring provides minimum standard consid-erations for eDNA and environmental RNA projects across the complete workflow, from ethical considerations and experimental design to interpreting and communicat-ing results. The Environmental DNA test validation guidelines outline key steps to be used in assay development and validation for species-specific testing and metabar-coding. Both guidelines were developed as an initiative of the Australian Government Department of Agriculture, Fisheries and Forestry and led by the Southern eDNA Society in a collaborative process including multiple consultation rounds with eDNA experts, end-users, and stakeholders to adapt the guidelines to Australian and New Zealand needs. The aim of these guidelines is not to be prescriptive, but to set mini-mum standards to support a consistent and best- practice approach to eDNA testing. We anticipate that the guidelines will be reviewed and regularly updated as required. Our aspiration is that these best- practice guidelines will ensure environmental man-agers are provided with robust scientific evidence to support decision- making.

Item ID:	77802
Item Type:	Article (Research - C1)
ISSN:	2637-4943
Keywords:	assay development, biosecurity, environmental monitoring, metabarcoding, methods, quality control, standard operating procedures, standardization, test validation
Copyright Information:	© 2023 The Authors. Environmental DNA published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Date Deposited:	28 Feb 2023 02:14
FoR Codes:	41 ENVIRONMENTAL SCIENCES > 4104 Environmental management > 410404 Environmental management @ 40% 41 ENVIRONMENTAL SCIENCES > 4104 Environmental management > 410406 Natural resource management @ 30% 41 ENVIRONMENTAL SCIENCES > 4104 Environmental management > 410407 Wildlife and habitat management @ 30%
SEO Codes:	18 ENVIRONMENTAL MANAGEMENT > 1899 Other environmental management > 189999 Other environmental management not elsewhere classified @ 50% 19 ENVIRONMENTAL POLICY, CLIMATE CHANGE AND NATURAL HAZARDS > 1902 Environmental policy, legislation and standards > 190299 Environmental policy, legislation and standards not elsewhere classified @ 50%
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