Weissenborn, S.J., Neale, R., de Koning, M.N.C., Waterboer, T., Abeni, D., Bouwes Bavinck, J.N., Wieland, U., Pfister, H.J., and EPI-HPV-UV-CA group, (2009) Prevalence and multiplicity of cutaneous beta papilloma viruses in plucked hairs depend on cellular DNA input. Journal of Virological Methods, 161 (2). pp. 280-283.

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Abstract

In view of the low loads of beta human papillomaviruses in skin samples, amounts of cellular DNA used in qualitative PCR may become limiting for virus detection and introduce variations in prevalence and multiplicity. This issue was explored within the context of a multicentre study and increasing prevalence and multiplicity was found with increasing input amounts of cellular DNA extracted from hair bulbs. To improve the quality and comparability between different epidemiologic studies ideally equal amounts of cellular DNA should be employed. When cellular DNA input varies this should be clearly taken into account in assessing viral prevalence and multiplicity.

Item ID:	77401
Item Type:	Article (Research - C1)
ISSN:	1879-0984
Keywords:	HPV; Human papillomavirus; Beta papillomavirus; PCR; Cellular DNA; Epidemiology; Detection; Prevalence; Multiplicity
Copyright Information:	© 2009 Elsevier B.V. All rights reserved.
Additional Information:	The EPI-HPV-UV-CA group includes JCU affiliated author, Simone Harrison.
Date Deposited:	28 Mar 2024 06:16
FoR Codes:	42 HEALTH SCIENCES > 4202 Epidemiology > 420299 Epidemiology not elsewhere classified @ 100%
SEO Codes:	20 HEALTH > 2004 Public health (excl. specific population health) > 200412 Preventive medicine @ 100%
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