In vitro degradation and biocompatibility of vitamin C loaded Ca-P coating on a magnesium alloy for bioimplant applications

Wang, Xue Mei, Lu, Guan Jie, Cui, Lan Yue, Liu, Cheng Bao, Kannan, M. Bobby, Zhang, Fen, Li, Shuo Qi, Zou, Yu Hong, and Zeng, Rong Chang (2022) In vitro degradation and biocompatibility of vitamin C loaded Ca-P coating on a magnesium alloy for bioimplant applications. Corrosion Communications, 6. pp. 16-28.

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Molecular recognition was utilized to fabricate bioinspired calcium phosphate (Ca-P) coating on bioabsorbable magnesium alloys through small biomolecules such as Vitamin C (VC). Ca-P and VC hybrid coating (Ca-PVC) was successfully fabricated on AZ31 Mg alloy. The surface morphology and chemical composition of the coatings were investigated using SEM, XRD, and FTIR together with XPS. The results showed that the Ca-PVC coating was composed of bamboo leaf-like Ca-P particles with a thickness of about three times that of the Ca-P coating. The surface roughness of the Ca-PVC coating (1.12 ± 0.12 µm) was lower than that (3.14 ± 1.93 µm) of Ca-P coating, suggesting the formation of refined Ca-P particles resulting from the VC addition. The corrosion resistance of the coated samples was characterized via electrochemical polarization, impedance spectroscopy, and immersion hydrogen evolution tests. The cell toxicity of the coated samples was evaluated utilizing mouse MC3T3-E1 pre-osteoblasts. The charge transfer resistance (Rct) of the Ca-PVC coated alloy increased as compared to the bare and Ca-P coated alloy samples. The Ca-PVC coated alloy exhibited minimal corrosion current density (1.36 × 10−6 A cm−2), which is one order of magnitude lower in comparison to that of the Ca-P coated alloy. These results confirm that VC addition greatly enhanced the coating resistance on AZ31 Mg alloy. It was also noticed that the Ca-PVC coated samples rapidly induced the formation of apatite after immersion in Hank's solution. VC was mainly transformed to L-Threonic acid, which facilitated the nucleation process of the Ca-PVC coating and significantly increased the thickness, density, and bonding strength of the coating. With enhanced corrosion resistance property and excellent biocompatibility, Ca-PVC coating has great potential for application in biodegradable Mg-based alloys.

Item ID: 76507
Item Type: Article (Research - C1)
ISSN: 2667-2669
Keywords: Biomaterial, Ca-P coating, Degradation, Magnesium alloy, Vitamin C
Copyright Information: © 2022 The Author(s). Published by Elsevier B.V. on behalf of Institute of Metal Research, Chinese Academy of Sciences. This is an open access article under the CC BY-NC-ND license (
Date Deposited: 27 Apr 2023 01:48
FoR Codes: 40 ENGINEERING > 4003 Biomedical engineering > 400302 Biomaterials @ 100%
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