Quantitative Assessment of the Efficacy of Two Different Single-file Systems in Reducing the Bacterial load in Oval-Shaped Canals: A Clinical Study

Rodrigues Amaral, Rodrigo, Guimarães Oliveira, Anna Gabriella, Braga, Tiago, Reher, Peter, de Macêdo Farias, Luiz, Magalhães, Paula Prazeres, Ferreira, Pedro Goncalves, and de Souza Côrtes, Maria Ilma (2020) Quantitative Assessment of the Efficacy of Two Different Single-file Systems in Reducing the Bacterial load in Oval-Shaped Canals: A Clinical Study. Journal of Endodontics, 46 (9). pp. 1228-1234.

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Abstract

Introduction: This randomized clinical study compared the in vivo antibacterial efficacy of Reciproc Blue (RB), XP-endo Shaper (XP-S), and XP-endo Shaper associated with XP-endo Finisher (XP-F) systems in infected oval-shaped root canals with primary apical periodontitis.

Methods: In this study, 28 human teeth with a single root and a single canal were randomly assigned to 2 groups according to the instrumentation technique: group 1, RB (n = 14) and group 2, XP-endo (XP-S and XP-F, n = 14). The single-rooted teeth were prepared by reciprocating and rotary nickel-titanium instruments with 5.25% sodium hypochlorite irrigation. Samples were collected from the canal at the baseline (S1), after chemomechanical preparation (S2), and after XP-F instrumentation (S3). The DNA extracts were subjected to quantitative analysis for total bacterial counts by quantitative real-time polymerase chain reaction. The data were analyzed using the analysis of variance test, and the level of significance was set at 5%.

Results: All samples tested positive for the presence of bacteria at baseline, and the bacterial counts substantially reduced after treatment procedures (P <.01). The results showed no statistical difference between RB and XP-S instrumentation with respect to the bacterial reduction (P >.05). A marked bacterial reduction was observed after the use of the XP-F instrument (P <.01).

Conclusions: The XP-S and RB systems sharply reduced the bacterial load in oval-shaped root canals with primary apical periodontitis. XP-F used as a supplementary instrument to chemomechanical preparation promoted a significantly higher bacterial reduction.

Item ID: 76142
Item Type: Article (Research - C1)
ISSN: 1878-3554
Keywords: Apical periodontitis, bacterial reduction, endodontic treatment, quantitative real-time polymerase chain reaction
Copyright Information: Copyright © 2020 American Association of Endodontists.
Date Deposited: 18 Oct 2022 03:46
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3203 Dentistry > 320304 Endodontics @ 100%
SEO Codes: 20 HEALTH > 2099 Other health > 209999 Other health not elsewhere classified @ 100%
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