Lipogenic gene single nucleotide polymorphic DNA markers associated with intramuscular fat, fat melting point, and health-beneficial omega-3 long-chain polyunsaturated fatty acids in Australian pasture-based Bowen Genetics Forest Pastoral Angus, Hereford and Wagyu beef cattle
Otto, John R., Mwangi, Felista W., Pewan, Shedrach B., Adegboye, Oyelola A., and Malau-Aduli, Aduli E.O. (2022) Lipogenic gene single nucleotide polymorphic DNA markers associated with intramuscular fat, fat melting point, and health-beneficial omega-3 long-chain polyunsaturated fatty acids in Australian pasture-based Bowen Genetics Forest Pastoral Angus, Hereford and Wagyu beef cattle. Genes, 13 (8). 1411.
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Abstract
This study used targeted sequencing aimed at identifying single nucleotide polymorphisms (SNP) in lipogenic genes and their associations with health-beneficial omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA), intramuscular fat (IMF), and fat melting point (FMP) of the M. longissimus dorsi muscle in Australian pasture-based Bowen Genetics Forest Pastoral Angus, Hereford, and Wagyu cattle. It was hypothesized that SNP encoding for the fatty acid-binding protein 4 (FABP4), stearoyl-CoA desaturase (SCD), and fatty acid synthase (FASN) genes will be significantly associated with health-beneficial n-3 LC-PUFA and the meat eating quality traits of IMF and FMP in an Australian pasture-based beef production system. Two SNP mutations, g.21267406 T>C and g.21271264 C>A, in the SCD gene were significantly (p < 0.05) associated with IMF, FMP, oleic acid (18:1n-9), linoleic acid (LA) 18:2n-6, alpha-linolenic acid (ALA) 18:3n-3, eicosapentaenoic acid (EPA) 20:5n-3, docosahexaenoic acid (DHA) 22:6-n-3, and docosapentaenoic acid (DPA) 22:5n-3. Significant positive correlations (p < 0.05) between FASN SNP g. 50787138 A>G and FMP, 18:1n-9, ALA, EPA, DHA, DPA, and total n-3 LC-PUFA were also detected. An SNP (g.44678794 G>A) in the FABP4 gene was associated with FMP. These results provide significant insights into the contributions of lipogenic genes to intramuscular fat deposition and the biosynthesis of health-beneficial n-3 LC-PUFA. The findings also unravel the potential use of lipogenic gene polymorphisms in marker-assisted selection to improve the content of health-promoting n-3 LC-PUFA and meat eating quality traits in Australian pasture-based Bowen Genetics Forest Pastoral Angus, Hereford, and Wagyu beef cattle.
| Item ID: | 75701 |
|---|---|
| Item Type: | Article (Research - C1) |
| ISSN: | 2073-4425 |
| Copyright Information: | Copyright: © 2022 by the author. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license https://creativecommons.org/license s/by/4.0/). |
| Date Deposited: | 10 Aug 2022 23:40 |
| FoR Codes: | 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3003 Animal production > 300302 Animal management @ 50% 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3003 Animal production > 300301 Animal growth and development @ 50% |
| SEO Codes: | 10 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 1004 Livestock raising > 100401 Beef cattle @ 100% |
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