The alternative splicing landscape of a coral reef fish during a marine heatwave

Chan, Stanley Kin Nok, Suresh, Sneha, Munday, Philip, Ravasi, Timothy, Bernal, Moisés A., and Schunter, Celia (2022) The alternative splicing landscape of a coral reef fish during a marine heatwave. Ecology and Evolution, 12 (3). e8738.

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Abstract

Alternative splicing is a molecular mechanism that enables a single gene to encode multiple transcripts and proteins by post-transcriptional modification of pre-RNA molecules. Changes in the splicing scheme of genes can lead to modifications of the transcriptome and the proteome. This mechanism can enable organisms to respond to environmental fluctuations. In this study, we investigated patterns of alternative splicing in the liver of the coral reef fish Acanthochromis polyacanthus in response to the 2016 marine heatwave on the Great Barrier Reef. The differentially spliced (DS; n = 40) genes during the onset of the heatwave (i.e., 29.49°C or +1°C from average) were related to essential cellular functions such as the MAPK signaling system, Ca(2+) binding, and homeostasis. With the persistence of the heatwave for a period of one month (February to March), 21 DS genes were detected, suggesting that acute warming during the onset of the heatwave is more influential on alternative splicing than the continued exposure to elevated temperatures. After the heatwave, the water temperature cooled to ~24.96°C, and fish showed differential splicing of genes related to cyto-protection and post-damage recovery (n = 26). Two-thirds of the DS genes detected across the heatwave were also differentially expressed, revealing that the two molecular mechanisms act together in A. polyacanthus to cope with the acute thermal change. This study exemplifies how splicing patterns of a coral reef fish can be modified by marine heatwaves. Alternative splicing could therefore be a potential mechanism to adjust cellular physiological states under thermal stress and aid coral reef fishes in their response to more frequent acute thermal fluctuations in upcoming decades.

Item ID: 74585
Item Type: Article (Research - C1)
ISSN: 2045-7758
Keywords: cellular physiology, climate change, molecular acclimation, thermal stress, transcriptome
Copyright Information: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. © 2022 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.
Date Deposited: 23 Nov 2022 00:01
FoR Codes: 31 BIOLOGICAL SCIENCES > 3105 Genetics > 310502 Cell and nuclear division @ 50%
31 BIOLOGICAL SCIENCES > 3104 Evolutionary biology > 310499 Evolutionary biology not elsewhere classified @ 25%
41 ENVIRONMENTAL SCIENCES > 4101 Climate change impacts and adaptation > 410102 Ecological impacts of climate change and ecological adaptation @ 25%
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