β-Glucan receptors on IL-4 activated macrophages are required for hookworm larvae recognition and trapping

Bouchery, Tiffany, Volpe, Beatrice, Doolan, Rory, Coakley, Gillian, Moyat, Mati, Esser-von Bieren, Julia, Wickramasinghe, Lakshanie C., Hibbs, Margaret L., Sotillo, Javier, Camberis, Mali, Le Gros, Graham, Khan, Nemat, Williams, David, and Harris, Nicola L. (2022) β-Glucan receptors on IL-4 activated macrophages are required for hookworm larvae recognition and trapping. Immunology and Cell Biology, 100 (4). pp. 223-234.

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Abstract

Recent advances in the field of host immunity against parasitic nematodes have revealed the importance of macrophages in trapping tissue migratory larvae. Protective immune mechanisms against the rodent hookworm Nippostrongylus brasiliensis (Nb) are mediated, at least in part, by IL-4-activated macrophages that bind and trap larvae in the lung. However, it is still not clear how host macrophages recognize the parasite. An in vitro co-culture system of bone marrow-derived macrophages and Nb infective larvae was utilized to screen for the possible ligand–receptor pair involved in macrophage attack of larvae. Competitive binding assays revealed an important role for β-glucan recognition in the process. We further identified a role for CD11b and the non-classical pattern recognition receptor ephrin-A2 (EphA2), but not the highly expressed β-glucan dectin-1 receptor, in this process of recognition. This work raises the possibility that parasitic nematodes synthesize β-glucans and it identifies CD11b and ephrin-A2 as important pattern recognition receptors involved in the host recognition of these evolutionary old pathogens. To our knowledge, this is the first time that EphA2 has been implicated in immune responses to a helminth.

Item ID: 74559
Item Type: Article (Research - C1)
ISSN: 1440-1711
Keywords: glucan, hookworms, macrophages, Nippostrongylus brasiliensis, recognition, trapping
Copyright Information: © 2022 The Authors. Immunology & Cell Biology published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology, Inc. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Funders: National Health and Medical Research Council of Australia (NHMRC)
Projects and Grants: NHMRC SRF-B fellowship
Date Deposited: 16 Nov 2022 02:05
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3207 Medical microbiology > 320704 Medical parasitology @ 100%
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