Rise of the terminator protein tus: A versatile tool in the biotechnologist's toolbox

Toft, Casey J., Sorenson, Alanna E., and Schaeffer, Patrick M. (2022) Rise of the terminator protein tus: A versatile tool in the biotechnologist's toolbox. Analytica Chimica Acta, 1213. 339946.

[img] PDF (Published Version) - Published Version
Restricted to Repository staff only

View at Publisher Website: https://doi.org/10.1016/j.aca.2022.33994...


Tus is a protein involved in DNA replication termination that binds specific DNA sequences (Ter) located around the terminus region of the chromosome in Enterobacterales. Tus and Ter form a unique monomeric protein-DNA complex which is one of strongest of its kind. A fascinating aspect of Tus-Ter is its ability to dramatically change conformation into a locked structure upon progression of a replication fork towards the non-permissive face of the complex. Over the last two decades, several new technologies have emerged harnessing the unique and interesting properties of this fascinating DNA-binding protein. This review highlights the important properties of the Tus-Ter complex and their exploitation for the development of diverse and novel ultrasensitive detection devices as well as innovative genomic and proteomic platform technologies. A variety of ex vivo and in vivo bioanalytical applications are discussed, including immuno-PCR diagnostic, bioassay and protein array technologies that are broadly relevant to the fields of cancer biology, microbiology and immunology. A perspective on future research and applications is provided.

Item ID: 74476
Item Type: Article (Research - C1)
ISSN: 1873-4324
Keywords: Immuno-PCR, Protease assay, Protein array, Replication fork barrier, Streptavidin-biotin, Tus-Ter
Copyright Information: © 2022 Elsevier B.V. All rights reserved.
Date Deposited: 26 Oct 2022 00:53
FoR Codes: 31 BIOLOGICAL SCIENCES > 3101 Biochemistry and cell biology > 310109 Proteomics and intermolecular interactions (excl. medical proteomics) @ 100%
Downloads: Total: 1
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page