Wiliams, David T., Paradkar, Prasad, and Karl, Stephan (2021) Arbovirus Detection in Vectors (Book Chapter). In: Tyagi, B.K, (ed.) Genetically Modified and other Innovative Vector Control Technologies: Eco-bio-social Considerations for Safe Application. Springer, Cham, Switzerland, pp. 277-295.

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Abstract

Abstract Detection of arthropod-borne (arbo)viruses is a fundamental element of mosquito surveillance programmes. Moreover, recent advances in modifying mosquitoes for managing arbovirus vector populations rely on sensitive arbovirus detection methods, which are applied at various stages of development, evaluation and production of modified mosquitoes. An increasingly wide range of mosquito trapping, sampling and testing options are available. Although virus culture will remain important for isolating viruses for research and reference purposes, the widespread use and application of real-time reverse transcription polymerase chain reaction (RT-PCR) offers rapid and cost-effective workflows for detecting arbovirus nucleic acid. Advances in next-generation sequencing (NGS) techniques and bioinformatics approaches have also enabled increasingly rapid, accurate and inexpensive arbovirus genome sequencing that can be employed following virus detection using conventional methods or used independently as a stand-alone platform. Unbiased NGS is also a powerful tool for arbovirus discovery and metagenomics. Continued advances in arbovirus detection methods and approaches are expected to provide ever more sophisticated tools for controlling and responding to the threat of pathogenic arboviruses.

Item ID:	72721
Item Type:	Book Chapter (Research - B1)
ISBN:	978-981-16-2963-1
Copyright Information:	© The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2021
Date Deposited:	11 May 2022 00:11
FoR Codes:	41 ENVIRONMENTAL SCIENCES > 4102 Ecological applications > 410202 Biosecurity science and invasive species ecology @ 100%
SEO Codes:	18 ENVIRONMENTAL MANAGEMENT > 1806 Terrestrial systems and management > 180602 Control of pests, diseases and exotic species in terrestrial environments @ 100%
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