Infectivity of Symptomatic Malaria Patients to Anopheles farauti Colony Mosquitoes in Papua New Guinea

Timinao, Lincoln, Vinit, Rebecca, Katusele, Michelle, Koleala, Tamarah, Nate, Elma, Czeher, Cyrille, Burkot, Thmoas R., Schofield, Louis, Felger, Ingrid, Mueller, Ivo, Laman, Moses, Robinson, Leanne J., and Karl, Stephan (2021) Infectivity of Symptomatic Malaria Patients to Anopheles farauti Colony Mosquitoes in Papua New Guinea. Frontiers in Cellular and Infection Microbiology, 11. 771233.

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Abstract

Plasmodium transmission from humans to mosquitoes is an understudied bottleneck in the transmission of malaria. Direct membrane feeding assays (DMFA) allow detailed malaria transmission studies from humans to mosquitoes. Especially for Plasmodium vivax, which cannot be cultured long-term under laboratory conditions, implementation of DMFAs requires proximity to P. vivax endemic areas. In this study, we investigated the infectivity of symptomatic Plasmodium infections to Anopheles farauti colony mosquitoes in Papua New Guinea (PNG). A total of 182 DMFAs were performed with venous blood collected from rapid diagnostic test (RDT) positive symptomatic malaria patients and subsequently analysed by light microscopy and quantitative real time polymerase chain reaction (qPCR). DMFAs resulted in mosquito infections in 20.9% (38/182) of cases. By light microscopy and qPCR, 10 – 11% of P. falciparum and 32 – 44% of P. vivax positive individuals infected An. farauti. Fifty-eight percent of P. vivax and 15% of P. falciparum gametocytaemic infections infected An farauti.

Item ID: 72719
Item Type: Article (Research - C1)
ISSN: 2235-2988
Copyright Information: Copyright © 2021 Timinao, Vinit, Katusele, Koleala, Nate, Czeher, Burkot, Schofield, Felger, Mueller, Laman, Robinson and Karl. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these term.
Funders: National Health and Medical Research Council of Australia (NHMRC)
Projects and Grants: NHMRC GNT1127356
Date Deposited: 12 Apr 2022 00:33
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3202 Clinical sciences > 320211 Infectious diseases @ 100%
SEO Codes: 20 HEALTH > 2001 Clinical health > 200101 Diagnosis of human diseases and conditions @ 100%
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