Identifying Epstein–Barr virus peptide sequences associated with differential IgG antibody response

Coghill, Anna E., Fang, Jianwen, Liu, Zhiwei, Chen, Chien-Jen, Jarrett, Ruth F., Hjalgrim, Henrik, Proietti, Carla, Yu, Kelly J., Hsu, Wan-Lun, Lou, Pei-Jen, Wang, Chen-Ping, Zhao, Yingdong, Doolan, Denise, and Hildesheim, Allan (2022) Identifying Epstein–Barr virus peptide sequences associated with differential IgG antibody response. International Journal of Infectious Diseases, 114. pp. 65-71.

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Background: Epstein-Barr virus (EBV) infection contributes to cancers in a fraction of seropositive individuals, but much remains to be learned about variation in EBV-directed humoral immunity in cancer-free adults.

Methods: A protein microarray was used to probe serum from 175 Taiwanese and 141 Northern European adults for immunoglobulin G (IgG) antibody responses to 115 different peptide sequences, representing protein segments or protein variants, from 45 EBV proteins. It was posited that this antibody-based approach could identify EBV peptide sequences representing immunodominant regions relevant for B-cell immunity.

Results: Analyses of 45 EBV proteins with multiple protein segments or variants printed on the array identified eight EBV peptide sequences that appear to play a role in immunogenicity. This included: (1) three proteins with segments/regions associated with IgG reactivity (BALF5, LMP1, LMP2A); and (2) five proteins with sequence variants/amino acid changes associated with IgG reactivity (BDLF4, EBNA3A, EBNA3B, EBNA-LP, LF1).

Conclusion: This examination of IgG antibody responses against 115 EBV peptide sequences in 316 cancer-free adults represents an important step toward identifying specific EBV protein sequences that play a role in generating B-cell immunity in humans.

Item ID: 72683
Item Type: Article (Research - C1)
ISSN: 1878-3511
Keywords: Epstein–Barr virus, IgG antibody array, LMP1/2, EBNA protein
Copyright Information: © 2021 Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY-NC-ND IGO license (
Date Deposited: 30 May 2022 01:57
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3207 Medical microbiology > 320705 Medical virology @ 100%
SEO Codes: 20 HEALTH > 2001 Clinical health > 200101 Diagnosis of human diseases and conditions @ 100%
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