Burkholderia pseudomallei enhances maturation of bone marrow derived dendritic cells

Williams, Natasha L., Kloeze, Eveline, Govan, Brenda L., Korner, Heinrich, and Ketheesan, Natkunam (2008) Burkholderia pseudomallei enhances maturation of bone marrow derived dendritic cells. Transactions of the Royal Society of Tropical Medicine and Hygiene, 102 (S1). pp. 71-75.

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T-cell activation is essential for protection against Burkholderia pseudomallei infection. Using bone marrow-derived dendritic cells (BMDC) isolated from partially resistant C57BL/6 and susceptible BALB/c mice, the degree of BMDC activation in the presence of B. pseudomallei was investigated. Maturation, cytokine production and internalization of B. pseudomallei by BMDC was assessed in response to infection with a highly virulent and a low-virulent clinical isolate. Maturation was determined by identifying the upregulation of cell-surface markers CD11c and CD86. IL-1b and IL-12p40 expression were assessed by reverse-transcriptase PCR. The uptake of B. pseudomallei by BMDC was measured using an internalization assay. This study demonstrated that B. pseudomallei isolates stimulate the maturation of BMDC to the same degree regardless of virulence. However, maturation of BMDC was significantly increased in BALB/c mice compared with C57BL/6 mice. Additionally, the uptake of B. pseudomallei by BMDC was significantly greater with the highly virulent isolate compared with the low-virulent isolate. Expression of IL-12 and IL-1b following infection with B. pseudomallei was up-regulated. The differences observed may have implications in the development of an effective immune response to B. pseudomallei.

Item ID: 7206
Item Type: Article (Research - C1)
ISSN: 0035-9203
Keywords: Burkholderia pseudomallei; dendritic cells; C57bL/6; maturation; internalisation
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This issue is Supplement 1 - Proceedings from the 5th World Melioidosis Congress.

Date Deposited: 15 Mar 2010 23:20
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1103 Clinical Sciences > 110309 Infectious Diseases @ 50%
11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110801 Medical Bacteriology @ 25%
11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110704 Cellular Immunology @ 25%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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