Ketheesan, Natkunam, Barnes, Jodie L., Ulett, Glen C., VanGessel, Helen J., Norton, Robert E., Hirst, Robert G., and LaBrooy, Justin T. (2002) Demonstration of a cell-mediated immune response in melioidosis. Journal of Infectious Diseases, 186 (2). pp. 286-289.

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Abstract

Melioidosis is a bacterial infection caused by Burkholderia pseudomallei. The aim of this study was to determine whether a cell-mediated adaptive immune response against B. pseudomallei developed in patients who had recovered from melioidosis. Lymphocyte proliferation assays were done on peripheral blood mononuclear cells from patients (n=13) and control subjects (n=10) to determine the lymphocyte response to B. pseudomallei antigens. Production of interferon-gamma and interleukin-10 was also determined. Activation of T cell subsets was assessed by fluorescence-activated cell sorter analysis, using antibodies to CD4, CD8, and CD69 antigens. Lymphocyte proliferation and interferon-gamma production in response to B. pseudomallei antigens were significantly higher (P<.001 for both) in patients than in control subjects. There was also an increase in the percentage of activated CD4+ (P<.004) and activated CD8+ T cells (P<.035) in cell cultures from patients. The development of such a cell-mediated immune response in patients may be essential for their survival.

PMID: 12134268 [PubMed - indexed for MEDLINE]

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