Poor protective potential of influenza nucleoprotein antibodies despite wide prevalence

Vanderven, Hillary A., Esterbauer, Robyn, Jegaskanda, Sinthujan, Tan, Hyon‐Xhi, Wheatley, Adam K., and Kent, Stephen J. (2022) Poor protective potential of influenza nucleoprotein antibodies despite wide prevalence. Immunology and Cell Biology, 100 (1). pp. 49-60.

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Abstract

Humans are exposed to influenza virus through periodic infections. Due to these repeated exposures, human populations commonly have elevated antibody titers targeting the conserved internal influenza virus nucleoprotein (NP). Despite the presence of anti-NP antibodies, humans are acutely susceptible to drifted influenza viruses with antigenically different surface proteins and the protective potential of human NP antibodies is unclear. In this study, high levels of anti-NP antibody and NP-specific B cells were detected in both adult humans and influenza-infected mice, confirming that NP is a major target of humoral immunity. Through sorting single B cells from influenza-exposed human adults, we generated a panel of 11 anti-NP monoclonal antibodies (mAbs). The majority of anti-NP human mAbs generated were capable of engaging cellular Fc receptors and bound NP on the surface of influenza-infected cell lines in vitro, suggesting that anti-NP mAbs have the potential to mediate downstream Fc effector functions such as antibody-dependent cellular cytotoxicity and antibody-dependent phagocytosis. However, human anti-NP mAbs were not protective in vivo when passively transferred into a murine influenza challenge model. Future in vivo studies examining the synergistic effect of anti-NP mAbs infused with other influenza-specific mAbs are warranted.

Item ID: 71143
Item Type: Article (Research - C1)
ISSN: 1440-1711
Keywords: antibody, humoral immunity, influenza virus, nucleoprotein
Copyright Information: © 2021 Australian and New Zealand Society for Immunology, Inc.
Funders: National Health and Medical Research Council of Australia (NHMRC)
Projects and Grants: NHMRC 1052979
Date Deposited: 15 Dec 2021 21:34
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3204 Immunology > 320405 Humoural immunology and immunochemistry @ 75%
32 BIOMEDICAL AND CLINICAL SCIENCES > 3204 Immunology > 320404 Cellular immunology @ 25%
SEO Codes: 20 HEALTH > 2099 Other health > 209999 Other health not elsewhere classified @ 100%
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