Exploring the evolution and epidemiology of european cc1mrsa-iv: Tracking a multidrug-resistant community-associated meticillin-resistant staphylococcus aureus clone

Earls, Megan R., Steinig, Eike J., Monecke, Stefan, Samaniego Castruita, José A., Simbeck, Alexandra, Schneider-Brachert, Wulf, Vremerǎ, Teodora, Dorneanu, Olivia S., Loncaric, Igor, Bes, Michèle, Lacoma, Alicia, Aymerich, Cristina Prat, Wernery, Ulrich, Armengol-Porta, Marc, Blomfeldt, Anita, Duchene, Sebastian, Bartels, Mette D., Ehricht, Ralf, and Coleman, David C. (2021) Exploring the evolution and epidemiology of european cc1mrsa-iv: Tracking a multidrug-resistant community-associated meticillin-resistant staphylococcus aureus clone. Microbial Genomics, 7 (7). 000601.

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Abstract

This study investigated the evolution and epidemiology of the community-associated and multidrug-resistant Staphylococcus aureus clone European CC1-MRSA-IV. Whole-genome sequences were obtained for 194 European CC1-MRSA-IV isolates (189 of human and 5 of animal origin) from 12 countries, and 10 meticillin-susceptible precursors (from North-Eastern Romania; all of human origin) of the clone. Phylogenetic analysis was performed using a maximum-likelihood approach, a time-measured phylogeny was reconstructed using Bayesian analysis, and in silico microarray genotyping was performed to identify resistance, virulence-associated and SCCmec (staphylococcal cassette chromosome mec) genes. Isolates were typically sequence type 1 (190/204) and spa type t127 (183/204). Bayesian analysis indicated that European CC1-MRSA-IV emerged in approximately 1995 before undergoing rapid expansion in the late 1990s and 2000s, while spreading throughout Europe and into the Middle East. Phylogenetic analysis revealed an unstructured meticillin-resistant S. aureus (MRSA) population, lacking significant geographical or temporal clusters. The MRSA were genotypically multidrug-resistant, consistently encoded seh, and intermittently (34/194) encoded an undisrupted hlb gene with concomitant absence of the lysogenic phage-encoded genes sak and scn. All MRSA also harboured a characteristic ~5350 nt insertion in SCCmec adjacent to orfX. Detailed demographic data from Denmark showed that there, the clone is typically (25/35) found in the community, and often (10/35) among individuals with links to South-Eastern Europe. This study elucidated the evolution and epidemiology of European CC1-MRSA-IV, which emerged from a meticillin-susceptible lineage prevalent in North-Eastern Romania before disseminating rapidly throughout Europe.

Item ID: 70659
Item Type: Article (Research - C1)
ISSN: 2057-5858
Keywords: CA-MRSA, Epidemiology, European CC1-MRSA-IV clone, Evolution, Phylogenomics, Transmission
Copyright Information: This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
Date Deposited: 03 May 2022 01:23
Downloads: Total: 110
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