Parallel murine and human aortic wall genomics reveals metabolic reprogramming as key driver of abdominal aortic aneurysm progression

Gäbel, Gabor, Northoff, Bernd H., Balboa, Amanda, Becirovic-Agic, Mediha, Petri, Marcelo, Busch, Albert, Maegdefessel, Lars, Mahlmann, Adrian, Ludwig, Stefan, Teupser, Daniel, de Waard, Vivian, Golledge, Jonathan, Wanhainen, Anders, Wågsäter, Dick, Holdt, Lesca M., and Lindeman, Jan H.N. (2021) Parallel murine and human aortic wall genomics reveals metabolic reprogramming as key driver of abdominal aortic aneurysm progression. Journal of the American Heart Association, 10 (17). e020231.

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BACKGROUND: While numerous interventions effectively interfered with abdominal aortic aneurysm (AAA) formation/progres-sion in preclinical models, none of the successes translated into clinical success. Hence, a systematic exploration of parallel and divergent processes in clinical AAA disease and its 2 primary models (the porcine pancreatic elastase and angiotensin-II infusion [AngII] murine model) was performed to identify mechanisms relevant for aneurysm disease.

METHODS AND RESULTS: This study combines Movat staining and pathway analysis for histological and genomic comparisons between clinical disease and its models. The impact of a notable genomic signal for metabolic reprogramming was tested in a rescue trial (AngII model) evaluating the impact of 1-(4-pyridinyl)-3-(2-quinolinyl)-2-propen-1-one (PFK15)-mediated interference with main glycolytic switch PFKFB3. Histological evaluation characterized the AngII model as a dissection model that is accompanied by adventitial fibrosis. The porcine pancreatic elastase model showed a transient inflammatory response and aortic dilatation, followed by stabilization and fibrosis. Normalization of the genomic responses at day 14 confirmed the self-limiting nature of the porcine pancreatic elastase model. Clear parallel genomic responses with activated adaptive immune responses, and particularly strong signals for metabolic switching were observed in human AAA and the AngII model. Rescue intervention with the glycolysis inhibitor PFK15 in the AngII model showed that interference with the glycolytic switching quenches aneurysm formation.

CONCLUSIONS: Despite clear morphological contrasts, remarkable genomic parallels exist for clinical AAA disease and the AngII model. The metabolic response appears causatively involved in AAA progression and provides a novel therapeutic target. The clear transient genomic response classifies the porcine pancreatic elastase model as a disease initiation model.

Item ID: 70132
Item Type: Article (Research - C1)
ISSN: 2047-9980
Keywords: Abdominal aortic aneurysm, Angiotensin II model, Elastase model, Gene expression, Glycolysis, Human, Metabolic reprogramming
Copyright Information: © 2021 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
Funders: National Health and Medical Research Council (NHMRC)
Projects and Grants: NHMRC (1020955), NHMRC (1021416), NHMRC (1063476), NHMRC (1000967)
Date Deposited: 23 Mar 2022 00:16
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