Genetic diversity and population structure of Haemonchus contortus in goats from Thailand

Pitaksakulrat, Opal, Chaiyasaeng, Monticha, Artchayasawat, Atchara, Eamudomkarn, Chatanun, Boonmars, Thidarut, Kopolrat, Kulthida Y., Prasopdee, Sattrachai, Petney, Trevor Neil, Blair, David, and Sithithaworn, Paiboon (2021) Genetic diversity and population structure of Haemonchus contortus in goats from Thailand. Infection, Genetics and Evolution, 95. 105021.

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Abstract

Haemonchus contortus is one of the most economically important parasitic nematodes affecting small ruminant livestock worldwide. This study was conducted to elucidate the genetic diversity and population structure of this nematode in Thailand based on mitochondrial DNA markers, the nicotinamide dehydrogenase subunit 4 (nad4) and the cytochrome c oxidase subunit 1 (cox1) genes. One hundred and thirty-six adult worms were obtained from 86 abomasa of slaughtered goats from 13 different localities in 5 regions of Thailand. Identification to the genus Haemonchus was done using morphology. DNA sequences of the nuclear ribosomal second internal transcribed spacer (ITS2) identified each specimen to species: three fixed nucleotide (SNP) differences distinguished H. contortus from H. placei. Genetic analysis defined 118 and 122 unique haplotypes in partial sequences of nad4 (alignment length 723 bp) and cox1 (645 bp) genes, respectively. Nucleotide diversities were 0.031 and 0.043 for nad4 and cox1 genes, respectively. Low genetic differentiation was observed among H. contortus samples from various provinces in Thailand. This is the first study on the genetic diversity and population structure of H. contortus of goats in Thailand. This study has provided insights into the transmission dynamics of this parasitic nematode, information which is essential for farm management and parasite control.

Item ID: 70025
Item Type: Article (Research - C1)
ISSN: 1567-7257
Keywords: Genetic diversity, Haemonchus contortus, Mitochondrial DNA, Population structure
Copyright Information: © 2021 Elsevier B.V. All rights reserved
Date Deposited: 29 Nov 2021 03:04
FoR Codes: 30 AGRICULTURAL, VETERINARY AND FOOD SCIENCES > 3009 Veterinary sciences > 300909 Veterinary parasitology @ 50%
31 BIOLOGICAL SCIENCES > 3104 Evolutionary biology > 310402 Biogeography and phylogeography @ 50%
SEO Codes: 10 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 1004 Livestock raising > 100405 Goats @ 50%
28 EXPANDING KNOWLEDGE > 2801 Expanding knowledge > 280101 Expanding knowledge in the agricultural, food and veterinary sciences @ 50%
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