Comparative analysis of two Himalayan Aconitum species for their phytopharmaceutical properties

Jamtsho, Tenzin, Yeshi, Karma, Samten, , and Wangchuk, Phurpa (2021) Comparative analysis of two Himalayan Aconitum species for their phytopharmaceutical properties. Journal of Herbal Medicine. 100497. (In Press)

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Abstract

Introduction: BhutaneseSowa Rigpa Medicine (BSM) uses two species of Aconitum in 25 important multi-ingredient formulations. The formulations are used for treating chronic parasitic, microbial infections, inflammatory conditions, bilious fever, or high fever related to bile disorders, including colds and flu. Some Aconitum species are toxic, and there is a need for stringent quality control measures.

Materials and methods: Aconitum orochryseum Stapf and Aconitum laciniatum (Bruehl) Stapf, were collected from Lingzhi, which is geographically located under Thimphu district, Bhutan, between the altitudes of 4000 4900 meters above sea level (masl), between July and September. Their comparative pharmacognostic properties were evaluated by: 1) using physicochemical parameters, such as foreign matter and moisture contents, total ash, acid-insoluble ash and alcohol-soluble extracts values and loss on drying of individual crude drugs; 2) developing a comparative High-Performance Thin Layer Chromatography (HPTLC) fingerprint, at Menjong Sorig Pharmaceuticals Corporation Limited (MSCPL), under the Ministry of Health, Bhutan and 3) conducting a literature review to identify the nature and toxicity of alkaloids present in two Aconitum species.

Results: Two Aconitum species possess distinct macro- and microscopic characteristic features (including colour, taste, smell, appearance, and shape). The physicochemical analysis and the HPTLC fingerprinting of their methanol extracts yielded adequate data to differentiate and validate these two species identities, before adding them to 25 essential traditional medicinal drugs (ETMDs) or polyherbal formulations in BSM. A literature review revealed that the two Aconitum species contain two distinct types of alkaloids.

Conclusions: Two Aconitum species could be differentiated based on their botanical features, macro- and microscopic characteristics, physicochemical values and HPTLC fingerprint profiles. These pharmacognostic properties can be used as quality control parameters to confirm the two species' identity, thereby ensuring the quality and safety of BSM.

Item ID: 69576
Item Type: Article (Research - C1)
ISSN: 2210-8033
Keywords: Bhutanese Sowa Rigpa Medicine, Aconitum, Pharmacognosy, Phytochemistry
Copyright Information: © 2020. Published by Elsevier.
Date Deposited: 26 Oct 2021 23:34
FoR Codes: 34 CHEMICAL SCIENCES > 3405 Organic chemistry > 340502 Natural products and bioactive compounds @ 30%
34 CHEMICAL SCIENCES > 3401 Analytical chemistry > 340107 Quality assurance, chemometrics, traceability and metrological chemistry @ 50%
34 CHEMICAL SCIENCES > 3401 Analytical chemistry > 340109 Separation science @ 20%
SEO Codes: 20 HEALTH > 2004 Public health (excl. specific population health) > 200412 Preventive medicine @ 50%
24 MANUFACTURING > 2408 Human pharmaceutical products > 240899 Human pharmaceutical products not elsewhere classified @ 50%
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