A comparison of two methods for the detection of circulating tumour cells in patients with oral cavity cancer

Curtin, Justin, Wong, Gordon, Wang, Weilan, Thomson, Peter, Lam, Alfred K., and Choi, Siu-Wai (2022) A comparison of two methods for the detection of circulating tumour cells in patients with oral cavity cancer. Journal of Oral Pathology and Medicine, 51 (2). pp. 249-255.

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Abstract

Background: Circulating tumour cells (CTCs) detected in patient blood samples are relevant as diagnostic and prognostic markers offering insights into tumour behaviour and guiding treatment of cancer at an individualised level. The aim of this study was to ascertain the feasibility of detecting CTCs in oral squamous cell carcinoma (OSCC) using two different methods so as to determine the optimal method for the study of this cancer.

Methods: Comparison of the numbers of CTCs, circulating tumour micro-emboli (CTMs) and circulating tumour endothelial cells (CTECs), was undertaken in forty clinical samples of oral squamous cell carcinoma (OSCC) determined by filtration (ISET®) and in situ fluorescent immunostaining (i-FISH, Cytelligen®) immunostaining and in situ hybridisation.

Results: i-FISH detected CTCs in 80% of samples compared with 40% of samples analysed by microfiltration. i-FISH detected CTCs in a further 40% of samples in which microfiltration did not detect CTCs. No CTC clusters were detected by microfiltration while i-FISH detected CTM in 12.5% of samples. i-FISH analysis detected CTECs in 20/40 samples.

Conclusion: These results highlight significant differences in detection of CTCs, CTM and CTECs between i-FISH and microfiltration when applied to OSCC samples, suggesting that technologies capable of detecting circulating aneuploid cells more accurately detect CTCs. i-FISH also detected CTM and CTEC not detected using ISET®. With proven prognostic relevance in adenocarcinomas, accurate enumeration of CTCs, CTMs and CTECs may be a clinically useful tool in the management of OSCC and may aid in the reduction of false-negative diagnoses.

Item ID: 69509
Item Type: Article (Research - C1)
ISSN: 1600-0714
Keywords: circulating tumour cells, fluorescence in situ hybridisation, ISET oral cancer
Copyright Information: © 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
Funders: Health and Medical Research Fund, Hong Kong (HMRF-HK)
Projects and Grants: HMRF-HK grant number 07182416
Date Deposited: 09 Nov 2021 23:07
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3203 Dentistry > 320305 Oral and maxillofacial surgery @ 50%
32 BIOMEDICAL AND CLINICAL SCIENCES > 3211 Oncology and carcinogenesis > 321107 Liquid biopsies @ 50%
SEO Codes: 20 HEALTH > 2001 Clinical health > 200101 Diagnosis of human diseases and conditions @ 50%
20 HEALTH > 2001 Clinical health > 200105 Treatment of human diseases and conditions @ 50%
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