Infection, dissemination, and replication of urban and sylvatic strains of dengue virus type 2 (Flaviviridae: Flavivirus) in Australian Aedes aegypti (Diptera: Culicidae)

Hall-Mendelin, Sonja, Pyke, Alyssa T., Ramirez, Ana L., Staunton, Kyran M., Buttonclay, Peter, McMahon, Jamie, Barcelon, Jean, and van den Hurk, Andrew F. (2021) Infection, dissemination, and replication of urban and sylvatic strains of dengue virus type 2 (Flaviviridae: Flavivirus) in Australian Aedes aegypti (Diptera: Culicidae). Journal of Medical Entomology, 58 (3). pp. 1412-1418.

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Abstract

The dengue viruses (DENVs) occur throughout tropical and subtropical regions of the world where they infect 100s of millions of people annually. In Australia, the dengue receptive zone is confined to the northern state of Queensland where the principal vector Aedes aegypti (L.) is present. In the current study, two populations of Ae. aegypti from north Queensland were exposed to two urban outbreak strains and one sylvatic strain of dengue virus type 2 (DENV-2). The titer of virus required to infect 50% of mosquitoes was between 105 and 106 50% tissue culture infectious dose (TCID)50/ml and was influenced by the combination of the origin of Ae. aegypti population and virus strain. When exposed to infectious bloodmeal titers > 106 TCID50/ml, infection and dissemination rates were all > 50% and were significantly affected by the origin of the mosquito population but not by the strain of DENV-2. Replication of DENV-2 was also significantly affected by the mosquito population and the titer of the infectious bloodmeal that mosquitoes were exposed to. The results of this study are discussed in the context of DENV transmission dynamics in northern Australia and the relative fitness of the sylvatic virus strain in urban Ae. aegypti populations.

Item ID: 68780
Item Type: Article (Research - C1)
ISSN: 1938-2928
Keywords: dengue virus type 2, sylvatic dengue virus, Australian Aedes aegypti, infection, replication
Copyright Information: © The Author(s) 2021. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Date Deposited: 25 Jul 2021 23:19
FoR Codes: 42 HEALTH SCIENCES > 4206 Public health > 420699 Public health not elsewhere classified @ 100%
SEO Codes: 20 HEALTH > 2004 Public health (excl. specific population health) > 200499 Public health (excl. specific population health) not elsewhere classified @ 100%
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