Measuring the host-seeking ability of Aedes aegypti destined for field release

Lau, Meng-Jia, Endersby-harshman, Nancy M., Axford, Jason K., Ritchie, Scott A., Hoffmann, Ary A., and Ross, Perran A. (2020) Measuring the host-seeking ability of Aedes aegypti destined for field release. American Journal of Tropical Medicine and Hygiene, 102 (1). pp. 223-231.

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Abstract

Host seeking is an essential process in mosquito reproduction. Field releases of modified mosquitoes for population replacement rely on successful host seeking by female mosquitoes, but host-seeking ability is rarely tested in a realistic context. We tested the host-seeking ability of female Aedes aegypti mosquitoes using a semi-field system. Females with different Wolbachia infection types (wMel-, wAlbB-infected, and uninfected) or from different origins (laboratory and field) were released at one end of a semi-field cage and recaptured as they landed on human experimenters 15 m away. Mosquitoes from each population were then identified with molecular tools or through minimal dusting with fluorescent powder. Wolbachia-infected and uninfected populations had similar average durations to landing and overall recapture proportions, as did laboratory and field-sourced Ae. aegypti. These results indicate that the host-seeking ability of mosquitoes is not negatively affected by Wolbachia infection or long-term laboratory maintenance. This method provides an approach to study the host-seeking ability of mosquitoes in a realistic setting, which will be useful when evaluating strains of mosquitoes that are planned for releases into the field to suppress arbovirus transmission.

Item ID: 67353
Item Type: Article (Research - C1)
ISSN: 1476-1645
Copyright Information: Copyright © 2020 by The American Society of Tropical Medicine and Hygiene.
Funders: National Health and Medical Research Council of Australia (NHMRC), Wellcome Trust
Projects and Grants: NHMRC 1132412, NHMRC 1118640
Date Deposited: 25 May 2021 00:21
FoR Codes: 42 HEALTH SCIENCES > 4202 Epidemiology > 420203 Environmental epidemiology @ 100%
Downloads: Total: 1
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