AmAMP1 from Acropora millepora and damicornin define a family of coral-specific antimicrobial peptides related to the Shk toxins of sea anemones

Mason, B., Cooke, I., Moya, A., Augustin, R., Lin, M.F., Satoh, N., Bosch, T.C.G., Bourne, D.G., Hayward, D.C., Andrade, N., Forêt, S., Ying, H., Ball, E.E., and Miller, D.J. (2021) AmAMP1 from Acropora millepora and damicornin define a family of coral-specific antimicrobial peptides related to the Shk toxins of sea anemones. Developmental and Comparative Immunology, 114. 103866.

[img] PDF (Published Version) - Published Version
Restricted to Repository staff only

View at Publisher Website: https://doi.org/10.1016/j.dci.2020.10386...
 
1
1


Abstract

A candidate antimicrobial peptide (AmAMP1) was identified by searching the whole genome sequence of Acropora millepora for short (<125AA) cysteine-rich predicted proteins with an N-terminal signal peptide but lacking clear homologs in the SwissProt database. It resembled but was not closely related to damicornin, the only other known AMP from a coral, and was shown to be active against both Gram-negative and Gram-positive bacteria. These proteins define a family of AMPs present in corals and their close relatives, the Corallimorpharia, and are synthesised as preproproteins in which the C-terminal mature peptide contains a conserved arrangement of six cysteine residues. Consistent with the idea of a common origin for AMPs and toxins, this Cys motif is shared between the coral AMPs and the Shk neurotoxins of sea anemones. AmAMP1 is expressed at late stages of coral development, in ectodermal cells that resemble the “ganglion neurons” of Hydra, in which it has recently been demonstrated that a distinct AMP known as NDA-1 is expressed.

Item ID: 67011
Item Type: Article (Research - C1)
ISSN: 1879-0089
Keywords: Antimicrobial peptides, Coral, CSαβ fold, Cysteine-rich proteins, Shk-type toxins
Copyright Information: © 2020 Elsevier Ltd. All rights reserved.
Funders: Australian Research Council (ARC)
Projects and Grants: ARC DP170104734, ARC CE14100020
Date Deposited: 31 Mar 2022 00:31
Downloads: Total: 1
Last 12 Months: 1
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page