Antioxidant phytoconstituents from Onosma bracteata Wall. (Boraginaceae) ameliorate the CCl4 induced hepatic damage: in vivo study in male wistar rats

Kumar, Ajay, Kaur, Varinder, Pandit, Kritika, Tuli, Hardeep Singh, Sak, Katrin, Jain, Subheet Kumar, and Kaur, Satwinderjeet (2020) Antioxidant phytoconstituents from Onosma bracteata Wall. (Boraginaceae) ameliorate the CCl4 induced hepatic damage: in vivo study in male wistar rats. Frontiers in Pharmacology, 11. 1301.

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Abstract

Onosma bracteata Wall. (Boraginaceae) is a highly valuable medicinal herb that is used for the treatment of fever, bronchitis, asthma, rheumatism, stomach irritation, and other inflammatory disorders. The present study aims to explore the hepatoprotective potential of ethanolic extract (Obeth) from O. bracteata aerial parts against carbon tetrachloride (CCl4) which causes hepatic damage in the male Wistar rats. Obeth showed effective radical quenching activity with an EC50 of 115.14 and 199.33 µg/mL in superoxide radical scavenging and lipid peroxidation analyses respectively along with plasmid DNA protective potential in plasmid nicking assay. The Obeth modulated mutagenicity of 2 Aminofluorine (2AF) in the pre-incubation mode of investigation (EC50 10.48 µg/0.1 mL/plate) in TA100 strain of Salmonella typhimurium. In in vivo studies, pretreatment of Obeth (50, 100, and 200 mg/kg) had the potential to normalize the biochemical markers aggravated by CCl4 (1mL/kg b.wt.) including liver antioxidative enzymes. Histopathological analysis also revealed the restoration of CCl4-induced liver histopathological alterations. Immunohistochemical studies showed that the treatment of Obeth downregulated the expression levels of p53 and cyclin D in hepatocytes. and downregulation in the Western blotting analysis revealed the downregulation of p-NF-kB, COX-2, and p53. HPLC data analysis showed the supremacy of major compounds namely, catechin, kaempferol, epicatechin, and Onosmin A in Obeth. The present investigation establishes the hepatoprotective and chemopreventive potential of O. bracteata against CCl4-induced hepatotoxicity via antioxidant defense system and modulation of the expression of proteins associated with the process of carcinogenesis in hepatic cells.

Item ID: 66745
Item Type: Article (Research - C1)
ISSN: 1663-9812
Keywords: antimutagenicity, apoptosis, carcinogenesis, hepatoprotective effect, Onosma bracteata, phytoconstituents
Copyright Information: Copyright © 2020 Kumar, Kaur, Pandit, Tuli, Sak, Jain and Kaur. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Funders: University Grants Commission (UGC) - Basic Scientific Research (BSR), DST-FIST
Projects and Grants: UGC grant no. R/FST/LSI-691/2016(C), UGC DRS V grant no. 4-13/2015/DRS-II (SAP-II)
Date Deposited: 21 May 2021 05:24
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3214 Pharmacology and pharmaceutical sciences > 321499 Pharmacology and pharmaceutical sciences not elsewhere classified @ 100%
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