Wolbachia genome stability and mtDNA variants in Aedes aegypti field populations eight years after release

Huang, Bixing, Yang, Qiong, Hoffmann, Ary A., Ritchie, Scott A., van den Hurk, Andrew F., and Warrilow, David (2020) Wolbachia genome stability and mtDNA variants in Aedes aegypti field populations eight years after release. iScience, 23 (10). 101572.

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Abstract

A dengue suppression strategy based on release of Aedes aegypti mosquitoes infected with the bacterium Wolbachia pipientis is being trialed in many countries. Wolbachia inhibits replication and transmission of dengue viruses. Questions remain regarding the long-term stability of virus-suppressive effects. We sequenced the Wolbachia genome and analyzed Ae. aegypti mitochondrial DNA markers isolated from mosquitoes sampled 2–8 years after releases in the greater Cairns region, Australia. Few changes were detected when Wolbachia genomes of field mosquitoes were compared with Wolbachia genomes of mosquitoes obtained soon after initial releases. Mitochondrial variants associated with the initial Wolbachia release stock are now the only variants found in release sites, highlighting maternal leakage as a possible explanation for rare Wolbachia-negative mosquitoes and not migration from non-release areas. There is no evidence of changes in the Wolbachia genome that indicate selection against its viral-suppressive effects or other phenotypes attributable to infection with the bacterium.

Item ID: 66669
Item Type: Article (Research - C1)
ISSN: 2589-0042
Keywords: Biological Sciences, Entomology, Genomics, Parasitology, Virology
Copyright Information: Crown Copyright(C) 2020. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Funders: Forensic and Scientific Services of Australia (FSSA), National Health and Medical Research Council of Australia (NHMRC)
Projects and Grants: FSSA grant number RSS19-001, NHMRC grant number 1132412, NHMRC grant number 1118640
Date Deposited: 28 Apr 2021 03:01
FoR Codes: 42 HEALTH SCIENCES > 4203 Health services and systems > 420315 One health @ 100%
Downloads: Total: 40
Last 12 Months: 33
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