Transcriptional memory-like imprints and enhanced functional activity in gamma delta T cells following resolution of malaria infection

Kumarasingha, Rasika, Ioannidis, Lisa J., Abeysekera, Waruni, Studniberg, Stephanie, Wijesurendra, Dinidu, Mazhari, Ramin, Poole, Daniel P., Mueller, Ivo, Schofield, Louis, Hansen, Diana S., and Eriksson, Emily M. (2020) Transcriptional memory-like imprints and enhanced functional activity in gamma delta T cells following resolution of malaria infection. Frontiers in Immunology, 11. 582358.

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Abstract

Gamma delta T cells play an essential role in the immune response to many pathogens, including Plasmodium. However, long-lasting effects of infection on the gamma delta T cell population still remain inadequately understood. This study focused on assessing molecular and functional changes that persist in the gamma delta T cell population following resolution of malaria infection. We investigated transcriptional changes and memory-like functional capacity of malaria pre-exposed gamma delta T cells using a Plasmodium chabaudi infection model. We show that multiple genes associated with effector function (chemokines, cytokines and cytotoxicity) and antigen-presentation were upregulated in P. chabaudi-exposed gamma delta T cells compared to gamma delta T cells from naive mice. This transcriptional profile was positively correlated with profiles observed in conventional memory CD8(+) T cells and was accompanied by enhanced reactivation upon secondary encounter with Plasmodium-infected red blood cells in vitro. Collectively our data demonstrate that Plasmodium exposure result in "memory-like imprints" in the gamma delta T cell population and also promotes gamma delta T cells that can support antigen-presentation during subsequent infections.

Item ID: 66152
Item Type: Article (Research - C1)
ISSN: 1664-3224
Keywords: RNA-Seq, memory, Plasmodium, chabaudi, &#947, &#948, T cell
Copyright Information: © 2020 Kumarasingha, Ioannidis, Abeysekera, Studniberg, Wijesurendra, Mazhari, Poole, Mueller, Schofield, Hansen and Eriksson. This is an open-access article distributed under the terms of the Creative Commons Attribution License(CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Funders: National Health and Medical Research Council of Australia (NHMRC)
Projects and Grants: NHMRC APP106722, NHMRC #1043345
Date Deposited: 25 Nov 2020 08:06
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3204 Immunology > 320499 Immunology not elsewhere classified @ 100%
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