The use of insects as a bioassay for Penaeus merguiensis densovirus (PmergDNV)

La Fauce, Kathy A., and Owens, Leigh (2008) The use of insects as a bioassay for Penaeus merguiensis densovirus (PmergDNV). Journal of Invertebrate Pathology, 98 (1). pp. 1-6.

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The lack of available cell lines has hampered the study of viral diseases in crustaceans. This is particularly important for aquaculture which has been plagued by viral diseases since its rapid expansion to meet with the growing demand for seafood products. This study was designed to find an alternative bioassay to cell lines by investigating the use of insects as potential animal models for Penaeus merguiensis densovirus (PmergDNV). Acheta domesticus (house cricket) and Tenebrio molitor (mealworms) were challenged with approximately 1 × 106 virions of PmergDNV by inoculation. PmergDNV was detected in 20% of Tenebrio molitor and 86.6% of Acheta domesticus challenged with PmergDNV. During a subsequent time course experiment, there was a non significant increase in PmergDNV titres (104–5 virions), reaching a maximum peak at day 5 (106 copies). A threshold of PmergDNV DNA level equal to or greater than 103 virions was necessary for mortality in Acheta domesticus. As the inoculum increased from 103 DNA copies to 104, 105, 106, mortality increased from 20% to 60%, 80% and 100%, respectively. This is the first evidence that insects may be directly used to study viruses from crustaceans and concludes Acheta domesticus may be used as a potential model to study Penaeus merguiensis densovirus.

Item ID: 6612
Item Type: Article (Research - C1)
ISSN: 1096-0805
Keywords: Penaeus merguiensis densovirus; Acheta domesticus; Tenebrio molitor; host infection; viral threshold
Date Deposited: 25 Feb 2010 00:57
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 50%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070712 Veterinary Virology @ 50%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830199 Fisheries - Aquaculture not elsewhere classified @ 100%
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