Novel allergen discovery through comprehensive de novo transcriptomic analyses of five shrimp species

Karnaneedi, Shaymaviswanathan, Huerlimann, Roger, Johnston, Elecia B., Nugraha, Roni, Ruethers, Thimo, Taki, Aya C., Kamath, Sandip D., Wade, Nicholas M., Jerry, Dean R., and Lopata, Andreas L. (2021) Novel allergen discovery through comprehensive de novo transcriptomic analyses of five shrimp species. International Journal of Molecular Sciences, 22 (1). 32.

[img]
Preview
PDF
Download (5MB) | Preview
View at Publisher Website: https://www.mdpi.com/1422-0067/22/1/32
 
1
106


Abstract

Shellfish allergy affects 2% of the world's population and persists for life in most patients. The diagnosis of shellfish allergy, in particular shrimp, is challenging due to the similarity of allergenic proteins from other invertebrates. Despite the clinical importance of immunological cross-reactivity among shellfish species and between allergenic invertebrates such as dust mites, the underlying molecular basis is not well understood. Here we mine the complete transcriptome of five frequently consumed shrimp species to identify and compare allergens with all known allergen sources. The transcriptomes were assembled de novo, using Trinity, from raw RNA-Seq data of the whiteleg shrimp (Litopenaeus vannamei), black tiger shrimp (Penaeus monodon), banana shrimp (Fenneropenaeus merguiensis), king shrimp (Melicertus latisulcatus), and endeavour shrimp (Metapenaeus endeavouri). BLAST searching using the two major allergen databases, WHO/IUIS Allergen Nomenclature and AllergenOnline, successfully identified all seven known crustacean allergens. The analyses revealed up to 39 unreported allergens in the different shrimp species, including heat shock protein (HSP), alpha-tubulin, chymotrypsin, cyclophilin, beta-enolase, aldolase A, and glyceraldehyde-3-phosphate dehydrogenase (G3PD). Multiple sequence alignment (Clustal Omega) demonstrated high homology with allergens from other invertebrates including mites and cockroaches. This first transcriptomic analyses of allergens in a major food source provides a valuable resource for investigating shellfish allergens, comparing invertebrate allergens and future development of improved diagnostics for food allergy.

Item ID: 65721
Item Type: Article (Research - C1)
ISSN: 1422-0067
Keywords: allergy; prawn; tropomyosin; allergen; RNA-Seq
Copyright Information: © 2020 by the authors. Li-censee MDPI, Basel, Switzerland. Thisarticle is an open access article distributedunder the terms and conditions of theCreative Commons Attribution (CC BY)license (https://creativecommons.org/licenses/by/4.0/).
Funders: Centre for Food Allergy Research (CFAR), National Health and Medical Research Council (NHMRC), Australian Research Council (ARC)
Projects and Grants: NHMRC Peter Doherty Fellow (GNT1124143), NHMRC APP1086656, ARC IH130200013
Date Deposited: 17 Feb 2021 01:38
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3204 Immunology > 320401 Allergy @ 50%
31 BIOLOGICAL SCIENCES > 3102 Bioinformatics and computational biology > 310204 Genomics and transcriptomics @ 50%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920108 Immune System and Allergy @ 100%
Downloads: Total: 106
Last 12 Months: 82
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page