Uptake of Schistosoma mansoni extracellular vesicles by human endothelial and monocytic cell lines and impact on vascular endothelial cell gene expression

Kifle, Desalegn Woldeyohannes, Chaiyadet, Sujittra, Waardenberg, Ashley J., Wise, Ingrid, Cooper, Martha, Becker, Luke, Doolan, Denise L., Laha, Thewarach, Sotillo, Javier, Pearson, Mark S., and Loukas, Alex (2020) Uptake of Schistosoma mansoni extracellular vesicles by human endothelial and monocytic cell lines and impact on vascular endothelial cell gene expression. International Journal for Parasitology, 50 (9). pp. 685-696.

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Abstract

The ability of the parasitic blood fluke Schistosoma mansoni and other parasitic helminths to manipulate host biology is well recognised, but the mechanisms that underpin these phenomena are not well understood. An emerging paradigm is that helminths transfer their biological cargo to host cells by secretion of extracellular vesicles (EVs). Herein, we show that two populations of S. mansoni secreted EVs - exosome-like vesicles (ELVs) and microvesicles (MVs) - are actively internalised in two distinct human cell lines that reflect the resident cell types encountered by the parasite in vivo: human umbilical vein endothelial cells (HUVECs) and THP-1 monocytes. RNA-sequencing of HUVECs co-cultured with S. mansoni ELVs compared with untreated HUVECs revealed differential expression of genes associated with intravascular parasitism, including vascular endothelial contraction, coagulation, arachidonic acid metabolism and immune cell trafficking and signalling. Finally, we show that antibodies raised against recombinant tetraspanin (TSP) proteins from the surface of S. mansoni EVs significantly blocked EV uptake by both HUVECs and THP-1 monocytes whereas pre-immunisation antibodies did not. To our knowledge, this is the first evidence demonstrating the internalisation of secreted EVs from any helminth into vascular endothelial cells, providing novel insight into the potential mechanisms underlying host-schistosome interactions. The ability of anti-TSP antibodies to block vesicle uptake by host target cells further supports the potential of TSPs as promising antigens for an anti-fluke vaccine. It also suggests a potential mechanism whereby the current candidate human schistosomiasis vaccine, Sm-TSP-2, exerts its protective effect in animal models. (C) 2020 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.

Item ID: 64246
Item Type: Article (Research - C1)
ISSN: 0020-7519
Keywords: Schistosoma mansoni, Platyhelminth, Extracellular vesicles, Human umbilical vein endothelial cells, THP-1 human monocytes, Parasitism
Copyright Information: © 2020 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.
Funders: National Health and Medical Research Council (NHMRC), James Cook University (JCU)
Projects and Grants: NHMRC APP1132975, NHMRC APP1117504, NHMRC APP1137285
Date Deposited: 02 Sep 2020 07:40
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3207 Medical microbiology > 320704 Medical parasitology @ 100%
SEO Codes: 20 HEALTH > 2001 Clinical health > 200104 Prevention of human diseases and conditions @ 100%
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