Woodward, L.A., Arimitsu, H., Hirst, R., and Oguma, K. (2003) Expression of Hc subunits from Clostridium botulinum types C and D and their evaluation as candidate vaccine antigens in mice. Infection and Immunity, 71 (5). pp. 2941-2944.

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Abstract

Two proteins representing the heavy-chain subunits of botulinum neurotoxin types C and D were expressed in Escherichia coli, and their vaccine potential was evaluated. Mice were vaccinated with doses ranging from 0.5 to 10 µg and were challenged with 10 to 105 50% lethal doses of toxin. For the type C subunit protein, C50, two doses of 2 µg were required for full protection, while, for type D subunit protein, D50, two 1-µg doses were required. A bivalent vaccine consisting of a mixture of these two proteins also provided protection against both botulinum neurotoxin type C and type D challenge. Antibody levels in serum were determined by both enzyme-linked immunosorbent assays and serum neutralization assays.

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