Recognition and sensitive detection of CTCs using a controllable label-free electrochemical cytosensor

Zhang, HongFen, Liang, Fangmiao, Wu, Xuemei, Liu, Yang, and Chen, Anjia (2020) Recognition and sensitive detection of CTCs using a controllable label-free electrochemical cytosensor. Microchimica Acta, 187. 487.

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Abstract

An excellent atomic layer deposition (ALD) method was adopted for the controllable systhesis of a xFe2O3-nPt (or nPt-xFe2O3)-coated graphene nanostructure (xFe2O3-nPt@graphene). The produced nanomaterials have been characterized by transmission electron microscopy (TEM), cyclic voltammetry (CV), and X-ray photoelectron spectroscopy (XPS). It is shown that xFe2O3 and nPt were effectively tailored and deposited on the graphene. A simple, rapid, and sensitive electrochemical cytosensor based on the controllable nanomaterials was successfully developed for MCF-7 cells detection by combining the high affinity and specificity of an aptamer. The prepared cytosensor displays a linear response to MCF-7 in the concentration range 18 to 1.5 × 106 cell mL−1 with the detection limit of 6 cell mL−1 (at an S/N of 3). This cytosensor was applied to detect circulating tumor cells (CTCs) in patient blood and the results were satisfied. The experimental results indicate that the proposed controllable electrochemical cytosensor is highly-sensitive, and convenient for clinical detection of breast CTCs.

Item ID: 64048
Item Type: Article (Research - C1)
ISSN: 1436-5073
Keywords: Circulating tumor cells, Controllable nanomaterials, Electrochemical cytosensor
Copyright Information: (c) Springer-Verlag GmbH Austria, part of Springer Nature 2020
Funders: Natural Science Foundation of Shanxi Province (NSFSP), Startup Foundation for Doctors of Shanxi Medical University, Fund for Shanxi Key Subjects Construction (FSKSC)
Projects and Grants: NSFSP 201701D121023, NSFSP 201701D121141, FSKSC 2016
Date Deposited: 11 Aug 2020 22:28
FoR Codes: 03 CHEMICAL SCIENCES > 0301 Analytical Chemistry > 030102 Electroanalytical Chemistry @ 70%
03 CHEMICAL SCIENCES > 0306 Physical Chemistry (incl Structural) > 030604 Electrochemistry @ 30%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970103 Expanding Knowledge in the Chemical Sciences @ 90%
86 MANUFACTURING > 8615 Instrumentation > 861503 Scientific Instruments @ 10%
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