Interactions of osteoprogenitor cells with a novel zirconia implant surface

Munro, Thomas, Miller, Catherine M., Antunes, Elsa, and Sharma, Dileep (2020) Interactions of osteoprogenitor cells with a novel zirconia implant surface. Journal of Functional Biomaterials, 11 (3). 50.

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Background: This study compared the in vitro response of a mouse pre-osteoblast cell line on a novel sandblasted zirconia surface with that of titanium. Material and Methods: The MC3T3-E1 subclone 4 osteoblast precursor cell line was cultured on either sandblasted titanium (SBCpTi) or sandblasted zirconia (SBY-TZP). The surface topography was analysed by three-dimensional laser microscopy and scanning electron microscope. The wettability of the discs was also assessed. The cellular response was quantified by assessing the morphology (day 1), proliferation (day 1, 3, 5, 7, 9), viability (day 1, 9), and migration (0, 6, 24 h) assays. Results: The sandblasting surface treatment in both titanium and zirconia increased the surface roughness by rendering a defined surface topography with titanium showing more apparent nano-topography. The wettability of the two surfaces showed no significant difference. The zirconia surface resulted in improved cellular spreading and a significantly increased rate of migration compared to titanium. However, the cellular proliferation and viability noted in our experiments were not significantly different on the zirconia and titanium surfaces. Conclusions: The novel, roughened zirconia surface elicited cellular responses comparable to, or exceeding that, of titanium. Therefore, this novel zirconia surface may be an acceptable substitute for titanium as a dental implant material.

Item ID: 63806
Item Type: Article (Research - C1)
ISSN: 2079-4983
Keywords: dental implants; Yttria-tetragonal zirconia; titanium; osseointegration; surface properties; immunocytochemistry; cell attachment; cell migration
Copyright Information: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (
Funders: James Cook University
Date Deposited: 23 Aug 2020 23:20
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3203 Dentistry > 320302 Dental materials and equipment @ 40%
40 ENGINEERING > 4016 Materials engineering > 401601 Ceramics @ 30%
40 ENGINEERING > 4016 Materials engineering > 401605 Functional materials @ 30%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920113 Oro-Dental Disorders @ 70%
86 MANUFACTURING > 8610 Ceramics, Glass and Industrial Mineral Products > 861002 Ceramics @ 30%
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