The effect of smoke derivatives and carbon utilisation on symbiotic germination of the endangered Pterostylis despectans (Orchidaceae)

Ritmejeryte, Edita, Obvintseva, Anna, and Huynh, Tien (2018) The effect of smoke derivatives and carbon utilisation on symbiotic germination of the endangered Pterostylis despectans (Orchidaceae). Lankesteriana, 18 (3). pp. 167-175.

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Abstract

Orchids are highly dependent on exogenous nutritional sources and mycorrhizal associations to survive, particularly when challenged by extreme environmental stress such as bushfires that contribute significantly to its decline in nature. In this study, the effect of smoke derivatives and carbon utilisation was explored to improve germination and seedling establishment of an Australian endangered orchid, Pterostylis despectans (Lowly Greenhood) and its mycorrhizal fungi. Stored seeds were germinated in vitro with peloton-isolated fungal isolates with varying concentrations of smoke water (0-1.0 mL L-1) to simulate fire and sucrose as the carbon source (10 g L-1). Smoke water significantly increased germination, with advanced protocorms and robust seedlings produced. Sucrose inhibited germination such that protocorms and leafing was absent with sucrose inclusion. Fungal isolates were highly variable on its germination efficacy and tolerance to smoke water, highlighting the importance of fungal diversity and supports research-based conservation strategies to circumvent environmental challenges.

Item ID: 63627
Item Type: Article (Research - C1)
ISSN: 2215-2067
Keywords: In vitro culture, Mycorrhizae, Smoke water, Symbiotic germination
Copyright Information: © Universidad de Costa Rica, 2018. Licensed under a Creative Commons Attribution-NonCommercial-No Derivs 3.0 Costa Rica License.
Funders: Australian Orchid Foundation, Helen McPherson Smith Trust
Date Deposited: 06 Aug 2020 01:23
FoR Codes: 06 BIOLOGICAL SCIENCES > 0602 Ecology > 060208 Terrestrial Ecology @ 100%
SEO Codes: 96 ENVIRONMENT > 9613 Remnant Vegetation and Protected Conservation Areas > 961306 Remnant Vegetation and Protected Conservation Areas in Forest and Woodlands Environments @ 100%
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