CD8α- and Langerin-negative dendritic cells, but not Langerhans cells, act as principal antigen-presenting cells in leishmaniasis

Ritter, Uwe, Meißner, Anja, Scheidig, Christina, and Körner, Heinrich (2004) CD8α- and Langerin-negative dendritic cells, but not Langerhans cells, act as principal antigen-presenting cells in leishmaniasis. European Journal of Immunology, 34 (6). pp. 1542-1550.

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In the early phase of leishmaniasis three types of potential antigen-presenting cells, including epidermal Langerhans cells (LC), dermal dendritic cells (DC) and inflammatory DC, are localizedat the site of infection. Therefore, it has been a central question which cell type is responsible for the initiation of a protective immune response. In the early stage of an anti-Leishmania immune response, detectable Leishmania major antigen was localized in the paracortex of the draining lymph nodes (LN). Characterization of antigen-positive cells showed that L. major co-localized with DC of a CD11c+ CD8α- Langerin- phenotype. To determine the area of antigen uptake, dermis or epidermis, and to further define the type of antigen-transporting cells, L. major was inoculated subcutaneously and concurrently LC were mobilized with fluorescein isothiocyanate (FITC). After 3 days, DC carrying L. major antigenwere always FITC-, indicating a dermal and not an epidermal origin. Moreover, addition of L. major antigen to ex vivo isolated CD8α- and CD8+ DC fromthe draining LN of L. major-infected C57BL/6 mice demonstrated that both DC subpopulations were able to stimulate antigen-specific T cell proliferation in vitro. Without addition of exogenous antigen only the CD8α- Langerin- DC were capable of stimulating antigen-specific T cell proliferation. Thus, we demonstrate that CD8α- Langerin- DC and not LC are the basis of the protective immune response to intracellular L. major parasites in vivo.

Item ID: 6310
Item Type: Article (Research - C1)
ISSN: 1524-4141
Keywords: antigens; Langerhans; Leishmania; Langerhans cells; parasitic protozoan; antigen presentation
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Date Deposited: 10 Feb 2010 01:21
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110704 Cellular Immunology @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920108 Immune System and Allergy @ 100%
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