Mini tryptophanyl-tRNA synthetase is required for a synthetic phenotype in vascular smooth muscle cells induced by IFN-gamma-mediated beta 2-adrenoceptor signaling

Biros, Erik, and Moran, Corey S. (2020) Mini tryptophanyl-tRNA synthetase is required for a synthetic phenotype in vascular smooth muscle cells induced by IFN-gamma-mediated beta 2-adrenoceptor signaling. Cytokine, 127. 154940.

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Abstract

Phenotypic modulation of vascular smooth muscle cells (AoSMCs) between quiescent 'contractile' and active 'synthetic' states is crucial in response to normal stimuli and pathological stressors. Previous studies have revealed the ability of interferon gamma (IFN-gamma) to activate and promote a synthetic phenotype in AoSMCs that parallels marked up-regulation of truncated tryptophanyl-tRNA synthetase (mini-TrpRS). Here we provide evidence to support an essential dependency of IFN-gamma-induced activation and synthetic phenotype in AoSMC on mini-TrpRS. This is based upon change in AoSMC morphology from epithelioid (active synthetic) to spindle-shaped (quiescent contractile) cells and expression of proteins and genes important in mediating or regulating contractile function of AoSMCs, following blockade of mini-TrpRS induced by IFN-gamma, via targeted siRNA or the decoy cognate amino acid D-Tryptophan.

Item ID: 62560
Item Type: Article (Research - C1)
ISSN: 1096-0023
Keywords: Tryptophanyl-tRNA synthetase, Vascular smooth muscle cells, Interferon gamma, Beta-2 adrenergic receptor
Copyright Information: (C) Elsevier Ltd.
Funders: James Cook University (JCU)
Projects and Grants: JCU College of Medicine and Dentistry
Date Deposited: 18 Mar 2020 07:32
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3299 Other biomedical and clinical sciences > 329999 Other biomedical and clinical sciences not elsewhere classified @ 100%
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