Cryo-archiving of Batrachochytrium dendrobatidis and other chytridiomycetes

Boyle, D.G., Hyatt, A.D., Daszak, P., Berger, L., Longcore, J.E., Porter, D., Hengstberger, S.G., and Olsen, V. (2003) Cryo-archiving of Batrachochytrium dendrobatidis and other chytridiomycetes. Diseases of Aquatic Organisms, 56 (1). pp. 59-64.

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Abstract

Batrachochytrium dendrobatidis is a major pathogen of frogs worldwide. It has been associated with catastrophic declines of frog populations including those in pristine habitats in Queensland, Australia. To facilitate genetic and disease studies of this fungus and related species, it is essential to have a reliable long-term storage method to maintain genetic integrity of isolates. We have adapted well-established techniques used for the long-term storage of tissue-culture cell lines to the preservation of B. dendrobatidis and other chytridiomycetes. This simple method has allowed us to recover these fungi from storage at -80°C and in liquid nitrogen over an extended period. With this technique it is now possible to preserve saprobic and parasitic isolates from a variety of environmental and disease situations for comparative genetic and biological studies.

Item ID: 6198
Item Type: Article (Research - C1)
ISSN: 1616-1580
Keywords: amphibian declines; Batrachochytrium dendrobatidis; chytrids; cryopreservation; storage
Date Deposited: 29 Mar 2010 23:20
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070707 Veterinary Microbiology (excl Virology) @ 100%
SEO Codes: 96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960899 Flora, Fauna and Biodiversity of Environments not elsewhere classified @ 100%
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