Malaria surveillance from both ends: concurrent detection of Plasmodium falciparum in saliva and excreta harvested from Anopheles mosquitoes

Ramírez, Ana L., van den Hurk, Andrew F., Mackay, Ian M., Yang, Annie S.P., Hewitson, Glen R., McMahon, Jamie L., Boddey, Justin A., Ritchie, Scott A., and Erickson, Sara M. (2019) Malaria surveillance from both ends: concurrent detection of Plasmodium falciparum in saliva and excreta harvested from Anopheles mosquitoes. Parasites and Vectors, 12. 355.

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Background: Malaria is the most important vector-borne disease in the world. Epidemiological and ecological studies of malaria traditionally utilize detection of Plasmodium sporozoites in whole mosquitoes or salivary glands by microscopy or serological or molecular assays. However, these methods are labor-intensive, and can over- or underestimate mosquito transmission potential. To overcome these limitations, alternative sample types have been evaluated for the study of malaria. It was recently shown that Plasmodium could be detected in saliva expectorated on honey-soaked cards by Anopheles stephensi, providing a better estimate of transmission risk. We evaluated whether excretion of Plasmodium falciparum nucleic acid by An. stephensi correlates with expectoration of parasites in saliva, thus providing an additional sample type for estimating transmission potential. Mosquitoes were exposed to infectious blood meals containing cultured gametocytes, and excreta collected at different time points post-exposure. Saliva was collected on honey-soaked filter paper cards, and salivary glands were dissected and examined microscopically for sporozoites. Excreta and saliva samples were tested by real time polymerase chain reaction (RT-rtPCR).

Results: Plasmodium falciparum RNA was detected in mosquito excreta as early as four days after ingesting a bloodmeal containing gametocytes. Once sporogony (the development of sporozoites) occurred, P. falciparum RNA was detected concurrently in both excreta and saliva samples. In the majority of cases, no difference was observed between the Ct values obtained from matched excreta and saliva samples, suggesting that both samples provide equally sensitive results. A positive association was observed between the molecular detection of the parasites in both samples and the proportion of mosquitoes with sporozoites in their salivary glands from each container. No distinguishable parasites were observed when excreta samples were stained and microscopically analyzed.

Conclusions: Mosquito saliva and excreta are easily collected and are promising for surveillance of malaria-causing parasites, especially in low transmission settings or in places where arboviruses co-circulate.

Item ID: 61761
Item Type: Article (Research - C1)
ISSN: 1756-3305
Keywords: Anopheles stephensi, excreta, malaria, mosquito, Plasmodium falciparum, saliva, sporozoite
Copyright Information: © 2019 The Author(s).
Funders: Queensland Government (QG), Walter and Eliza Hall Institute, National Health and Medical Research Council, Victorian State Government Operational Infrastructure Support, Australian Government NHMRC IRIISS, James Cook University (JCU), National Health and Medical Research Council (NHMRC)
Projects and Grants: QG Australian Institute of Tropical Health and Medicine Capacity Building Grant, NHMRC Project grant 1049811, JCU doctoral scholarship, NHMRC research fellowships 1123727 and 1044698
Date Deposited: 17 May 2020 21:30
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3207 Medical microbiology > 320704 Medical parasitology @ 100%
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