Photoelectrochemical and electrochemical ratiometric aptasensing: a case study of streptomycin

Shen, Xiuli, Liu, Dong, Zhu, Chengxi, Li, Yuye, Liu, Yang, and You, Tianyan (2020) Photoelectrochemical and electrochemical ratiometric aptasensing: a case study of streptomycin. Electrochemistry Communications, 110. 106637.

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There has been much interest in constructing ratiometric sensors using different sensing techniques because of their synergistic effect, although the simultaneous collection of the signals is challenging. Herein, we propose a ratiometric aptasensing strategy based on the dual-detection model with a photoelectrochemical (PEC) “signalon” and an electrochemical (EC) “signal-off”. As a proof-of-concept study, CdTe quantum dots (CdTe QDs) and a methylene blue-labeled aptamer (MB-Apt) were used to generate PEC and EC signals in the sensing system. The target-induced conformational change of MB-Apt pushed MB away from the electrode, thereby decreasing the EC signal; at the same time, the reduced steric hindrance favored the restoration of the PEC signal from the CdTe QDs. Thus, this PEC-EC strategy can achieve the PEC “signal-on” and EC “signal-off” states simultaneously, as well as allowing quantitative analysis of the target based on the ratio of the current intensities. As a model application, an aptasensor fabricated for streptomycin detection showed a wide linear range from 0.03 to 100 μM with a detection limit of 10 nM (S/N = 3). The proposed sensing platform displayed superior analytical properties compared with methods based on PEC or EC alone. Our work provides an efficient dual-detection modelbased ratiometric strategy for advanced analysis, and paves the way to the simultaneous acquisition of signals.

Item ID: 61272
Item Type: Article (Research - C1)
ISSN: 13882481
Keywords: ratiometric strategy; dual-detection model; photoelectrochemistry; electrochemistry; aptasensor
Copyright Information: © 2019 The Authors. Published by Elsevier B.V. This is an Open Access article under the CC BY-NC-ND license.
Funders: National Natural Science Foundation of China (NNSF), Natural Science Foundation of Jiangsu Province of China (NSFJPC), Innovation/Entrepreneurship Program of Jiangsu Province, Priority Academic Program Development of Jiangsu Higher Education Institutions, Postgraduate Research & Practice Innovation Program of Jiangsu Province
Projects and Grants: NNSF grant no. 21675065, NNSF grant no. 6191193, NSFJPC No. BK20160490, PAPD-2018-87, KYCX19_1618
Date Deposited: 15 Jan 2020 01:10
FoR Codes: 34 CHEMICAL SCIENCES > 3401 Analytical chemistry > 340103 Electroanalytical chemistry @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970103 Expanding Knowledge in the Chemical Sciences @ 100%
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