A volume-pressure tail cuff method for hemodynamic parameters: comparison of restraint and light isoflurane anesthesia in normotensive male Lewis rats

Hem, Nur-Aish, Phie, James, Chilton, Lisa, and Kinobe, Robert (2019) A volume-pressure tail cuff method for hemodynamic parameters: comparison of restraint and light isoflurane anesthesia in normotensive male Lewis rats. Journal of Pharmacological and Toxicological Methods, 100. 106601.

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A volume-pressure sensor and tail-cuff method for monitoring blood pressure is non-invasive and inexpensive. This method requires animals to be restrained or subjected to anesthesia, but comparative effects of these manipulations on hemodynamic parameters have not been documented.


Using a volume-pressure sensor and tail-cuff, we serially measured blood pressure and heart rate in normotensive adult male Lewis rats after light isoflurane-induced anesthesia (5% induction, 1% maintenance) and, following untrained restraint. Blood pressure was recorded until the acquisition of three complete measurements without the range of replicate mean arterial pressures exceeding 15 mmHg (steady-state).


Averages for the entire series of consecutive measurements indicated that restraint yielded significantly higher systolic and diastolic blood pressure than anesthesia (P < .05), but heart rate was not affected. Following stabilization at steady-state, there were no significant differences in intra- or inter-day hemodynamic values between the restraint and isoflurane groups. The inter-day coefficient of variation for systolic pressure was 13–23% for isoflurane and 9–14% for restraint. Bland-Altman analysis showed wide limits of agreement (±59 mmHg systolic; ±49 mmHg diastolic pressure) between restraint and isoflurane measurements.


Isoflurane caused more variability but there was agreement in BP evaluation by the isoflurane and restraint methods. Using the VPR system, light isoflurane-induced anesthesia and restraint could effectively be used to screen and quantify overt changes in hemodynamic parameters for cardiovascular research utilizing laboratory rodents.

Item ID: 60623
Item Type: Article (Research - C1)
ISSN: 1873-488X
Keywords: Tail-cuff; Blood pressure measurement; Isoflurane and restraint methods; Systolic and diastolic blood pressure
Copyright Information: © 2019 Elsevier Inc. All rights reserved.
Funders: James Cook University (JCU)
Projects and Grants: JCU Internal research grant AITHM-2140.12005.0130
Date Deposited: 16 Oct 2019 00:50
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3201 Cardiovascular medicine and haematology > 320199 Cardiovascular medicine and haematology not elsewhere classified @ 50%
32 BIOMEDICAL AND CLINICAL SCIENCES > 3214 Pharmacology and pharmaceutical sciences > 321499 Pharmacology and pharmaceutical sciences not elsewhere classified @ 50%
SEO Codes: 92 HEALTH > 9299 Other Health > 929999 Health not elsewhere classified @ 100%
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