Omega-3 fatty acids decrease oxidative stress and inflammation in macrophages from patients with small abdominal aortic aneurysm

Meital, Lara T., Windsor, Mark T., Perissiou, Maria, Schulze, Karl, Magee, Rebecca, Kuballa, Anna, Golledge, Jonathan, Bailey, Tom G., Askew, Christopher D., and Russell, Fraser D. (2019) Omega-3 fatty acids decrease oxidative stress and inflammation in macrophages from patients with small abdominal aortic aneurysm. Scientific Reports, 9. 12978.

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Abstract

Abdominal aortic aneurysm (AAA) is associated with inflammation and oxidative stress, the latter of which contributes to activation of macrophages, a prominent cell type in AAA. Omega-3 polyunsaturated fatty acids (n-3 PUFAs) have been reported to limit oxidative stress in animal models of AAA. The aim of this study was to evaluate the effect of the n-3 PUFA docosahexaenoic acid (DHA) on antioxidant defence in macrophages from patients with AAA. Cells were obtained from men with small AAA (diameter 3.0-4.5 cm, 75 +/- 6yr, n = 19) and age- matched male controls (72 +/- 5 yr, n = 41) and incubated with DHA for 1 h before exposure to 0.1 mu g/mL lipopolysaccharide (LPS) for 24 h. DHA supplementation decreased the concentration of tumour necrosis factor-alpha. (TNF-alpha; control, 42.1 +/- 13.6 to 5.1 +/- 2.1 pg/ml, p < 0.01; AAA, 25.2 +/- 9.8 to 1.9 +/- 0.9 pg/ml, p < 0.01) and interleukin-6 (IL-6; control, 44.9 +/- 7.7 to 5.9 +/- 2.0 pg/ml, p < 0.001; AAA, 24.3 +/- 5.2 to 0.5 +/- 0.3 pg/ml, p < 0.001) in macrophage supernatants. DHA increased glutathione peroxidase activity (control, 3.2 +/- 0.3 to 4.1 +/- 0.2 nmol/min/ml/mu g protein, p= 0.004; AAA, 2.3 +/- 0.5 to 3.4 +/- 0.5 nmol/min/ml/mu g protein, p = 0.008) and heme oxygenase-1 mRNA expression (control, 1.5-fold increase, p < 0.001). The improvements in macrophage oxidative stress status serve as a stimulus for further investigation of DHA in patients with AAA.

Item ID: 60546
Item Type: Article (Research - C1)
ISSN: 2045-2322
Copyright Information: Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Funders: Sunshine Coast Health Foundation, University of the Sunshine Coast, National Health and Medical Research Council (NHMRC)
Projects and Grants: NHMRC 1117061
Date Deposited: 02 Oct 2019 07:32
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1102 Cardiovascular Medicine and Haematology > 110299 Cardiovascular Medicine and Haematology not elsewhere classified @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920199 Clinical Health (Organs, Diseases and Abnormal Conditions) not elsewhere classified @ 100%
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