A new model for standardising and treating thermal injury in the rat

Davenport, Lisa, Dobson, Geoffrey, and Letson, Hayley (2019) A new model for standardising and treating thermal injury in the rat. MethodsX, 6. pp. 2021-2027.

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Thermal burn injury methodologies are inconsistently described within the current literature. To permit the advancement of new treatments there is an urgent need for the development and standardisation of an acute rat model. We describe a rat thermal burn model that involves: anaesthesia, chronic catheterisation, skin preparation, baseline hemodynamic and physiological monitoring, and a quantifiable method to reproduce a severe full-thickness burns injury affecting ∼30% percent of the total body surface area (%TBSA). Following a 15 min post-burn period, treatment commences with an acute monitoring phase lasting up to 8 hours, which can be modified according to individual protocols. This model reflects the clinical continuum-of-care from point-of injury, a 15 min ambulance response time, a 60 min prehospital phase and hospital treatment monitoring phase. The model is validated with histological evidence of full-thickness injury, evidence of the hypermetabolic response (K+, Base Excess, lactate) and changes in complete blood counts.

• It has been 50 years since Walker and Mason published their widely popular a standard animal burn model.

• The model, however, lacks quantifiable methodology for the assessment of burn thickness, surface area burnt and physiological status.

• We present a new standardised method for evaluation of drug and interventional therapies that mimic the clinical scenario including ambulance response, pre-hospital and hospital phases after burn.

Item ID: 60397
Item Type: Article (Research - C1)
ISSN: 2215-0161
Keywords: burns; rat model; thermal injury; prehospital
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Copyright Information: © 2019. The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license(http://creativecommons.org/licenses/by/4.0/)
Funders: KJ Mcpherson Education and Research Foundation, Queensland Ambulance Service, James Cook University
Date Deposited: 02 Oct 2019 00:15
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3202 Clinical sciences > 320299 Clinical sciences not elsewhere classified @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920117 Skin and Related Disorders @ 30%
92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920199 Clinical Health (Organs, Diseases and Abnormal Conditions) not elsewhere classified @ 70%
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