Development of a tropical marine fish cell line from asian seabass (lates calcarifer) for virus isolation

Chang, S. F., Ngoh, G. H., Kueh, L. F. S., Qin, Q. W., Chen, C. L., Lam, T. J., and Sin, Y. M. (2001) Development of a tropical marine fish cell line from asian seabass (lates calcarifer) for virus isolation. Aquaculture, 192 (2-4). pp. 133-145.

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Abstract

A tropical marine fish cell line (SF) was established from Asian seabass (Lates calcarifer) fry. The cell line was maintained in Earle's minimum essential medium (EMEM) supplemented with foetal calf serum and incubated at 25°C. The susceptibility of the cell-line at the 16th, 35th and 46th subculture to several iridoviruses, birnaviruses, reoviruses, a rhabdovirus, and a nodavirus was studied. Cytopathic effect (CPE) was observed daily after virus inoculation and viral replication efficiency was determined for six viruses. SF cell cultures infected with an iridovirus, a nodavirus and a reovirus were further elucidated by electron microscopy. All the viruses tested were shown to induce CPE on SF cells. This suggests that the SF cell line has good potential for the isolation of various fish viruses. The SF cell line consists predominantly of epithelial-like cells and has been subcultured 85 times over a period of 24 months. © 2001 Elsevier Science B.V.

Item ID: 59131
Item Type: Article (Research - C1)
ISSN: 1873-5622
Keywords: Lates calcarifer, tropical marine fish cell line, virus
Copyright Information: Copyright © 2001 Elsevier Science B.V. All rights reserved.
Date Deposited: 26 Nov 2019 05:27
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070703 Veterinary Diagnosis and Diagnostics @ 50%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070712 Veterinary Virology @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 100%
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