Adaptation of the secretome of Echinostoma caproni may contribute to parasite survival in a Th1 milieu

Cortés, Alba, Muñoz-Antolí, Carla, Álvarez-Izquierdo, María, Sotillo, Javier, Esteban, J. Guillermo, and Toledo, Rafael (2018) Adaptation of the secretome of Echinostoma caproni may contribute to parasite survival in a Th1 milieu. Parasitology Research, 117 (4). pp. 947-957.

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Abstract

Echinostoma caproni (Trematoda: Echinostomatidae) is an intestinal trematode, broadly employed to study the host-dependent mechanisms that govern the evolution of intestinal helminth infections. Resistance against E. caproni homologous secondary infections has been reported in mice and appears to be related to the generation of a local Th2 response, whereas Th1 responses promote the development of chronic primary infections. Herein, the ability of E. caproni to modulate its secretome according to the host environment is investigated. A two-dimensional differential in gel electrophoresis (2D-DIGE) analysis was performed to elucidate changes in the excretory/secretory products of E. caproni adults after primary and secondary infections in mice. A total of 16 protein spots showed significant differences between groups, and 7 of them were successfully identified by mass spectrometry. Adult worms exposed to a primary infection appear to upregulate proteins involved in detoxification (aldo-keto reductase), stress response (GroEL), and enhancement of parasite survival (acetyl-CoA A-acetyltransferase and UTP-glucose-1-phosphate urydyltransferase). In contrast, any protein was found to be significantly upregulated after secondary infection. Upregulation of such proteins may serve to withstand the hostile Th1 environment generated in primary infections in mice. These results provide new insights into the resistance mechanisms developed by the parasites to ensure their long-term survival.

Item ID: 58484
Item Type: Article (Research - C1)
ISSN: 1432-1955
Keywords: 2D-DIGE; Echinostoma caproni; Immune response; Infection resistance; Secretome modulation; Trematoda
Copyright Information: © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
Funders: Ministerio de Economía y Competitividad (MEC), Conselleria d’Educació, Generalitat Valenciana (CEGV) Spain, Ministerio de Sanidad y Consumo (MSC)
Projects and Grants: MEC grant number BFU2016-75639-P, CEGV - grant number PROMETEO2014-083 Fase II, MSC No. RD12/0018/0013
Date Deposited: 03 Jun 2019 23:29
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 100%
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