Identification of Burkholderia pseudomallei by use of the Vitek mass spectrometer

Gassiep, I., Armstrong, M., and Norton, R.E. (2019) Identification of Burkholderia pseudomallei by use of the Vitek mass spectrometer. Journal of Clinical Microbiology, 57 (5). e00081-19.

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Abstract

Burkholderia pseudomallei is the causative agent of melioidosis. This condition most often presents as pneumonia and bacteremia, with mortality rates of 9% to 70%. Therefore, early identification of this organism may aid in directing appropriate management. This study aimed to use the Vitek matrix-assisted laser desorption ionization-time of flight mass spectrometer to create a spectrum for the rapid identification of B. pseudomallei. Spectra from 85 isolate cultures were acquired using the Vitek mass spectrometer research mode. A SuperSpectrum was created using peak matching and subsequently activated for analysis of organism identification. All 85 isolates were correctly identified as B. pseudomallei. A total of 899 spectra were analyzed and demonstrated a specificity of 99.8%. Eighty-one clinical isolates were used, of which 10 were neuromelioidosis, and no discernible spectrum difference was appreciated. Spectrum acquisition from a single spot was only successful in 374/899 (41%) of isolates. This increased to 100% when 3 spots of the same extract were analyzed. The Vitek mass spectrometer can be used for the rapid identification of B. pseudomallei with a high level of specificity.

Item ID: 58294
Item Type: Article (Research - C1)
ISSN: 1098-660X
Keywords: Burkholderia pseudomallei, matrix-assisted laser desorption ionization, melioidosis, diagnostics, mass spectrometer
Copyright Information: © 2019 American Society for Microbiology. All Rights Reserved.
Date Deposited: 15 May 2019 07:55
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110801 Medical Bacteriology @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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