Synthesis, crystal structure and biological evaluation of new phosphoramide derivatives as urease inhibitors using docking, QSAR and kinetic studies

Gholivand, Khodayar, Pooyan, Mahsa, Mohammadpanah, Fahimeh, Pirastefar, Foroogh, Junk, Peter C., Wang, Jun, Valmoozi, Ali Asghar Ebrahimi, and Mani-Varnosfaderani, Ahmad (2019) Synthesis, crystal structure and biological evaluation of new phosphoramide derivatives as urease inhibitors using docking, QSAR and kinetic studies. Bioorganic Chemistry, 86. pp. 482-493.

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Abstract

In an attempt to achieve a new class of phosphoramide inhibitors with high potency and resistance to the hydrolysis process against urease enzyme, we synthesized a series of bisphosphoramide derivatives (01-43) and characterized them by various spectroscopic techniques. The crystal structures of compounds 22 and 26 were investigated using X-ray crystallography. The inhibitory activities of the compounds were evaluated against the jack bean urease and were compared to monophosphoramide derivatives and other known standard inhibitors. The compounds containing aromatic amines and their substituted derivatives exhibited very high inhibitory activity in the range of IC50= 3.4-1.91x10(-10) nM compared with monophosphoramides, thiourea, and ;acetohydroxamic acid. It was also found that derivatives with P = O functional groups have higher anti-urease activity than those with P = S functional groups. Kinetics and docking studies were carried out to explore the binding mechanism that showed these compounds follow a mixed-type mechanism and, due to their extended structures, can cover the entire binding pocket of the enzyme, reducing the formation of the enzyme-substrate complex. The quantitative structure-activity relationship (QSAR) analysis also revealed that the interaction between the enzyme and inhibitor is significantly influenced by aromatic rings and P = O functional groups. Collectively, the data obtained from experimental and theoretical studies indicated that these compounds can be developed as appropriate candidates for urease inhibitors in this field.

Item ID: 58193
Item Type: Article (Research - C1)
ISSN: 1090-2120
Keywords: Bisphosphoramide derivatives, Urease enzyme, Inhibitory activity, Docking, Kinetics, QSAR
Copyright Information: © 2019 Published by Elsevier Inc. All rights reserved.
Funders: Tarbiat Modares University
Date Deposited: 01 May 2019 07:35
FoR Codes: 03 CHEMICAL SCIENCES > 0302 Inorganic Chemistry > 030202 f-Block Chemistry @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970103 Expanding Knowledge in the Chemical Sciences @ 100%
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