Characterization of tapeworm metabolites and their reported biological activities

Wangchuk, Phurpa, Constantinoiu, Constantin, Eichenberger, Ramon M., Field, Matt, and Loukas, Alex (2019) Characterization of tapeworm metabolites and their reported biological activities. Molecules, 24 (8). 1480.

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Parasitic helminths infect billions of people, livestock, and companion animals worldwide. Recently, they have been explored as a novel therapeutic modality to treat autoimmune diseases due to their potent immunoregulatory properties. While feeding in the gut/organs/tissues, the parasitic helminths actively release excretory-secretory products (ESP) to modify their environment and promote their survival. The ESP proteins of helminths have been widely studied. However, there are only limited studies characterizing the non-protein small molecule (SM) components of helminth ESP. In this study, using GC-MS and LC-MS, we have investigated the SM ESP of tapeworm Dipylidium caninum (isolated from dogs) which accidentally infects humans via ingestion of infected cat and dog fleas that harbor the larval stage of the parasite. From this D. caninum ESP, we have identified a total of 49 SM (35 polar metabolites and 14 fatty acids) belonging to 12 different chemotaxonomic groups including amino acids, amino sugars, amino acid lactams, organic acids, sugars, sugar alcohols, sugar phosphates, glycerophosphates, phosphate esters, disaccharides, fatty acids, and fatty acid derivatives. Succinic acid was the major small molecule present in the D. caninum ESP. Based on the literature and databases searches, we found that of 49 metabolites identified, only 12 possessed known bioactivities.

Item ID: 58145
Item Type: Article (Research - C1)
ISSN: 1420-3049
Keywords: helminths; tapeworm; excretory-secretory products; small molecules; bioactivities
Copyright Information: Copyright © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
Funders: National Health and Medical Research Council (NHMRC), Australian Institute of Tropical Health and Medicine (AITHM)
Projects and Grants: NHMRC Peter Doherty Early Career Fellowship, NHMRC program grant APP1037304, NHMRC Senior Principal Research Fellowship APP1117504, AITHM Capacity Development Grant
Date Deposited: 24 Apr 2019 04:16
FoR Codes: 32 BIOMEDICAL AND CLINICAL SCIENCES > 3205 Medical biochemistry and metabolomics > 320501 Medical biochemistry - amino acids and metabolites @ 40%
34 CHEMICAL SCIENCES > 3401 Analytical chemistry > 340101 Analytical spectrometry @ 30%
34 CHEMICAL SCIENCES > 3404 Medicinal and biomolecular chemistry > 340401 Biologically active molecules @ 30%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920120 Zoonoses @ 30%
97 EXPANDING KNOWLEDGE > 970103 Expanding Knowledge in the Chemical Sciences @ 30%
97 EXPANDING KNOWLEDGE > 970111 Expanding Knowledge in the Medical and Health Sciences @ 40%
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