Limited degradation of the Plasmodium falciparum gametocyte marker pfs25 mRNA exposed to tropical temperatures: considerations for malaria transmission field studies

Waltmann, Andreea, Karl, Stephan, Chiu, Chris, and Mueller, Ivo (2016) Limited degradation of the Plasmodium falciparum gametocyte marker pfs25 mRNA exposed to tropical temperatures: considerations for malaria transmission field studies. American Journal of Tropical Medicine and Hygiene, 94 (4). pp. 886-889.

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Abstract

An important aspect of many malaria molecular epidemiology and transmission studies is RNA -based detection of gametocytes. Ensuring RNA stability represents a challenge in tropical, resource -limited environments, as RNA may quickly degrade when samples are not preserved under adequate conditions. This study investigated the degradation of pfs25 messenger RNA (mRNA), the most widely used Plasmodium falciparum gametocyte marker, in whole blood spiked with cultured P falciparum gametocytes, exposed to different temperatures for up to 48 hours, and collected with different anticoagulants. The levels of pfs25 mRNA were similar between samples stored at 4 C and 30 C for up to 48 hours before stabilization with RNAprotect (Qiagen, Hilden, Germany). We observed that pfs25 mRNA in heparin -collected blood degraded less than that in ethylenediaminetetraacetic acid (EDTA)-collected blood over the 48 -hour period. For field studies aiming for P falciparum. gametocyte detection, immediate stabilization of blood samples is not necessary, as the pfs25 transcript is relatively stable, more so in heparin than EDTA collection tubes.

Item ID: 58071
Item Type: Article (Research - C1)
ISSN: 1476-1645
Copyright Information: Copyright © 2016 by The American Society of Tropical Medicine and Hygiene.
Date Deposited: 17 Apr 2019 09:24
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060502 Infectious Agents @ 50%
11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 50%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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