Identification of novel and zoonotic Cryptosporidium species in fish from Papua New Guinea

Koinari, M., Karl, S., Ng-Hublin, J., Lymbery, A.J., and Ryan, U.M. (2013) Identification of novel and zoonotic Cryptosporidium species in fish from Papua New Guinea. Veterinary Parasitology, 198 (1-2). pp. 1-9.

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There is still limited information on the distribution of Cryptosporidium species and genotypes in fish. The present study investigated the prevalence of Cryptosporidium species in cultured freshwater (n = 132), wild freshwater (n = 206) and wild marine (n = 276) fish in Papua New Guinea (PNG) by PCR screening at the 18S rRNA locus. A total of seven fish (2 cultured freshwater, 1 wild freshwater and 4 wild marine fish) were identified as positive for Cryptosporidium. Specifically, Cryptosporidium was found in four different host species (Nile tilapia, Oreochromis niloticus; silver barb, Puntius gonionotus; mackerel scad, Decapterus maracellus and oblong silver biddy, Gerres oblongus), giving an overall prevalence of 1.14% (95% CI: 0.3-2%, n = 7/614). Of the seven positive isolates, five were identified as C. parvum and two were a novel piscine genotype, which we have named piscine genotype 8. Piscine genotype 8 was identified in two marine oblong silver biddies and exhibited 4.3% genetic distance from piscine genotype 3 at the 18S locus. Further subtyping of C panvum isolates at the 60 kDa glycoprotein (gp60) locus identified 3 C. parvum subtypes (IlaA14G2R1, lIaA15G2R1 and IlaA19G4R1) all of which are zoonotic and a C. hominis subtype (IdA15G1). The zoonotic Gyptosporidium were identified in fish samples from all three groups; cultured and wild freshwater and wild marine fish. Detection of Cryptosporidium among aquaculture fingerlings warrants further research to gain a better understanding of the epidemiology of Cryptosporidium infection in cultured fish. The identification of zoonotic Cryptosporidium genotypes in fish from PNG has important public health implications and should be investigated further. (C) 2013 Elsevier B.V. All rights reserved.

Item ID: 58066
Item Type: Article (Research - C1)
ISSN: 1873-2550
Keywords: Cryptosporidium, Fish, 18S rRNA, gp60, Novel genotype, Zoonotic
Copyright Information: © 2013 Elsevier B.V. All rights reserved
Date Deposited: 17 Apr 2019 09:24
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060502 Infectious Agents @ 50%
11 MEDICAL AND HEALTH SCIENCES > 1117 Public Health and Health Services > 111715 Pacific Peoples Health @ 50%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920120 Zoonoses @ 100%
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