Phumrattanaprapin, Wuttipong, Chaiyadet, Sujittra, Loukas, Alex, Brindley, Paul J., Sotillo, Javier, and Laha, Thewarach (2018) Surface display on Bacillus subtilis spores and vaccine potential of a Tetraspanin fromm caniogenic liver fluke Opisthorchis viverrini. Southeast Asian Journal of Tropical Medicine and Public Health, 49 (6). pp. 933-948.

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Abstract

Opisthorchis viverrini resides in bile ducts and could be targeted optimally by vaccination, which induces both mucosal and systemic antibodies, reflecting the migratory path of this liver fluke. Tetraspanins are transmembrane proteins essential for tegument formation of O. viverrini and are efficacious as vaccine antigens for diseases caused by this and other parasitic flatworms. Tetraspanin-2 of O. viverrini (Ov-TSP-2) was expressed on the surface of Bacillus subtilis spores following transformation of B. subtilis strain WB800N with a plasmid encoding a fusion of a large extracellular loop region of Ov-TSP-2 (LEL-Ov-TSP-2) and B. subtilis spore coat protein CotC. Immunogenicity of the recombinant spores was assessed by measuring serum and bile immunoglobulins following oral vaccination of hamsters with the recombinant spores, 2.5x10(8) spores six times over four weeks. Sporulation of recombinant B. subtilis expressing fusion proteins was confirmed by western blotting and immunofluorescence using anti-Ov-TSP-2 antibodies. Significantly elevated levels of serum- and bile-specific IgG antibodies to LEL-Ov-TSP-2 were evident at day 42 following the first oral dosing. The findings indicate oral vaccination of hamster with recombinant B. subtilis spores expressing Ov-TSP-2 holds promise as a suitable approach for the control of this carcinogenic liver fluke infection.

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